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Preservation of Human Saphenous Vein against Reactive Oxygen Species-induced Oxidative Stress by Green Tea Polyphenol Pretreatment

 Dong-Wook Han  ;  Hwal Suh  ;  Jong-Chul Park  ;  Suong-Hyu Hyon  ;  Bum Koo Cho  ;  Young Hwan Park 
 ARTIFICIAL ORGANS, Vol.27(12) : 1137-1142, 2003 
Journal Title
Issue Date
Antioxidants/pharmacology* ; Endothelial Cells/drug effects* ; Flavonoids/pharmacology* ; Humans ; Models, Biological ; Oxidative Stress/drug effects* ; Phenols/pharmacology* ; Polyphenols ; Reactive Oxygen Species/adverse effects* ; Saphenous Vein/drug effects ; Tea ; Vascular Diseases/etiology ; Vascular Diseases/prevention & control
Reactive oxygen species ; Green tea polyphenol ; Human saphenous vein ; Endothelial cell ; Cellular viability
The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanthine oxidase in the presence of xanthine (0.5 mM). After incubation, the viability of the endothelial cells dissociated from veins and the histology of the veins were evaluated. Due to both types of treatment, a significant decrease in cellular viability, severe morphological changes in the veins, and extracellular structural damage were induced. The H2O2-induced alterations were prevented by preincubating the veins with either 0.5 or 1.0 mg/ml of GTPP for 1 h. When the oxidative stress was induced by xanthine oxidase, cellular viability and venous structure were preserved at the same polyphenol concentrations. These results demonstrate that GTPP can act as a biological antioxidant and protect veins from oxidative stress-induced toxicity.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Hwan(박영환) ORCID logo https://orcid.org/0000-0001-9802-8017
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
Cho, Bum Koo(조범구)
Han, Dong Wook(한동욱)
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