Purpose: The purpose of this study was to investigate the effects of BCAA and additional OKG or albumin supplements on fatigue factors and energy substrates changes in prolonged exercise. Methods: The subjects of this study were 5 male college students and the order of experiments was control, BCAA, BCAA+OKG, BCAA+ albumin. In each experiment, the subject ran for 90 minutes on the treadmill and exercise intensity was 3% gradient and 5mph. Blood was drawn five times; before the exercise, after 45 minutes of exercise, end of exercise, and 30minutes after ending of exercise. Blood was used to analyze ammonia, 5-HT, lactate, glucose and FFA. Results: The concentration of blood ammonia was low in the BCAA+OKG and BCAA+albumin groups compared to control and BCAA groups. And, lowest in the BCAA+albumin supplement group during exercise and recovery. The 5-HT concentration of control and BCAA supplement groups represented higher than BCAA+OKG and BCAA+albumin groups from resting to 45 min of exercise. However, at 90 min of exercise, BCAA+albumin group was highest, but BCAA+OKG was the lowest. The concentration of lactate was the highest in the BCAA supplement group, but represented low tendency in the BCAA+OKG and BCAA+albumin supplement group at the end of exercise and recovery. In glucose, control group was the highest, but BCAA+albumin supplement group was the lowest at the end of exercise and recovery. The concentration of FFA was the highest in the BCAA+albumin supplement group, but was the lowest in the BCAA supplement group at the 45 min and 90 min of exercise. Conclusion: As the result of this study was shown, BCAA and additional OKG or albumin can solve these problems that BCAA accumulate fatigue factors when it is oxidized. Furthermore, BCAA can be used as energy substrate when OKG or albumin is added to BCAA. Therefore, it is considered that BCAA and additional OKG or albumin supplement has effects on prolonged exercise. Specially, we are considered by thing which BCAA+albumin supplement is the most effect.