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The promoter of brain-specific angiogenesis inhibitor 1-associated protein 4 drives developmentally targeted transgene expression mainly in adult cerebral cortex and hippocampus

DC FieldValueLanguage
dc.contributor.author김미영-
dc.date.accessioned2015-07-14T17:29:58Z-
dc.date.available2015-07-14T17:29:58Z-
dc.date.issued2004-
dc.identifier.issn0014-5793-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/113005-
dc.description.abstractRestricting transgene expression to specific cell types and maintaining long-term expression are major goals for gene therapy. Previously, we cloned brain-specific angiogenesis inhibitor 1-associated protein 4 (BAI1-AP4), a novel brain-specific protein that interacts with BAI1, and found that it was developmentally upregulated in the adult brain. In this report, we isolated 5 kb of the 5′ upstream sequence of the mouse BAI1-AP4 gene and analyzed its promoter activity. Functional analyses demonstrated that an Sp1 site was the enhancer, and the region containing the transcription initiation site and an AP2-binding site was the basal promoter. We examined the ability of the BAI1-AP4 promoter to drive adult brain-specific expression by using it to drive lacZ expression in transgenic (TG) mice. Northern blot analyses showed a unique pattern of β-galactosidase expression in TG brain, peaking at 1 month after birth, like endogenous BAI1-AP4. Histological analyses demonstrated the same localization and developmental expression of β-galactosidase and BAI1-AP4 in most neurons of the cerebral cortex and hippocampus. Our data indicate that TG mice carrying the BAI1-AP4 promoter could be a valuable model system for region-specific brain diseases.-
dc.description.statementOfResponsibilityopen-
dc.format.extent87~94-
dc.relation.isPartOfFEBS LETTERS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAnimals, Newborn-
dc.subject.MESHBase Sequence-
dc.subject.MESHBinding Sites-
dc.subject.MESHCell Adhesion Molecules, Neuronal/genetics*-
dc.subject.MESHCerebral Cortex/cytology-
dc.subject.MESHCerebral Cortex/metabolism-
dc.subject.MESHCerebral Cortex/physiology*-
dc.subject.MESHGene Expression Regulation, Developmental-
dc.subject.MESHHippocampus/cytology-
dc.subject.MESHHippocampus/metabolism-
dc.subject.MESHHippocampus/physiology*-
dc.subject.MESHHumans-
dc.subject.MESHLac Operon/genetics-
dc.subject.MESHMice-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHNeurons/metabolism-
dc.subject.MESHPromoter Regions, Genetic/genetics-
dc.subject.MESHTissue Distribution-
dc.subject.MESHTranscription Initiation Site-
dc.subject.MESHTranscription, Genetic/genetics*-
dc.subject.MESHTransgenes-
dc.subject.MESHbeta-Galactosidase/metabolism-
dc.titleThe promoter of brain-specific angiogenesis inhibitor 1-associated protein 4 drives developmentally targeted transgene expression mainly in adult cerebral cortex and hippocampus-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorMi-Young Kim-
dc.contributor.googleauthorKyu Youn Ahn-
dc.contributor.googleauthorKyung Keun Kim-
dc.contributor.googleauthorKee Sook Lee-
dc.contributor.googleauthorChoon Sang Bae-
dc.contributor.googleauthorByeong Jo Chun-
dc.contributor.googleauthorJeong Tae Koh-
dc.contributor.googleauthorSeon Min Lee-
dc.identifier.doi10.1016/j.febslet.2004.03.106-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00446-
dc.relation.journalcodeJ00890-
dc.identifier.eissn1873-3468-
dc.identifier.pmid15147874-
dc.subject.keywordBAI1-AP4-
dc.subject.keywordbrain-specific angiogenesis inhibitor 1-associated protein 4-
dc.subject.keywordHSV-TK-
dc.subject.keywordherpes simplex virus-thymidine kinase-
dc.subject.keywordNSE-
dc.subject.keywordneuron-specific enolase-
dc.subject.keywordTG-
dc.subject.keywordtransgenic-
dc.subject.keywordBAI1-AP4 promoter-
dc.subject.keywordNeuron-specific expression-
dc.subject.keywordTransgene-
dc.subject.keywordDevelopmental expression-
dc.contributor.alternativeNameKim, Mi Young-
dc.contributor.affiliatedAuthorKim, Mi Young-
dc.rights.accessRightsfree-
dc.citation.volume566-
dc.citation.number1-3-
dc.citation.startPage87-
dc.citation.endPage94-
dc.identifier.bibliographicCitationFEBS LETTERS, Vol.566(1-3) : 87-94, 2004-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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