Cited 9 times in
The promoter of brain-specific angiogenesis inhibitor 1-associated protein 4 drives developmentally targeted transgene expression mainly in adult cerebral cortex and hippocampus
DC Field | Value | Language |
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dc.contributor.author | 김미영 | - |
dc.date.accessioned | 2015-07-14T17:29:58Z | - |
dc.date.available | 2015-07-14T17:29:58Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113005 | - |
dc.description.abstract | Restricting transgene expression to specific cell types and maintaining long-term expression are major goals for gene therapy. Previously, we cloned brain-specific angiogenesis inhibitor 1-associated protein 4 (BAI1-AP4), a novel brain-specific protein that interacts with BAI1, and found that it was developmentally upregulated in the adult brain. In this report, we isolated 5 kb of the 5′ upstream sequence of the mouse BAI1-AP4 gene and analyzed its promoter activity. Functional analyses demonstrated that an Sp1 site was the enhancer, and the region containing the transcription initiation site and an AP2-binding site was the basal promoter. We examined the ability of the BAI1-AP4 promoter to drive adult brain-specific expression by using it to drive lacZ expression in transgenic (TG) mice. Northern blot analyses showed a unique pattern of β-galactosidase expression in TG brain, peaking at 1 month after birth, like endogenous BAI1-AP4. Histological analyses demonstrated the same localization and developmental expression of β-galactosidase and BAI1-AP4 in most neurons of the cerebral cortex and hippocampus. Our data indicate that TG mice carrying the BAI1-AP4 promoter could be a valuable model system for region-specific brain diseases. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 87~94 | - |
dc.relation.isPartOf | FEBS LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Animals, Newborn | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Binding Sites | - |
dc.subject.MESH | Cell Adhesion Molecules, Neuronal/genetics* | - |
dc.subject.MESH | Cerebral Cortex/cytology | - |
dc.subject.MESH | Cerebral Cortex/metabolism | - |
dc.subject.MESH | Cerebral Cortex/physiology* | - |
dc.subject.MESH | Gene Expression Regulation, Developmental | - |
dc.subject.MESH | Hippocampus/cytology | - |
dc.subject.MESH | Hippocampus/metabolism | - |
dc.subject.MESH | Hippocampus/physiology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lac Operon/genetics | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | Neurons/metabolism | - |
dc.subject.MESH | Promoter Regions, Genetic/genetics | - |
dc.subject.MESH | Tissue Distribution | - |
dc.subject.MESH | Transcription Initiation Site | - |
dc.subject.MESH | Transcription, Genetic/genetics* | - |
dc.subject.MESH | Transgenes | - |
dc.subject.MESH | beta-Galactosidase/metabolism | - |
dc.title | The promoter of brain-specific angiogenesis inhibitor 1-associated protein 4 drives developmentally targeted transgene expression mainly in adult cerebral cortex and hippocampus | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Mi-Young Kim | - |
dc.contributor.googleauthor | Kyu Youn Ahn | - |
dc.contributor.googleauthor | Kyung Keun Kim | - |
dc.contributor.googleauthor | Kee Sook Lee | - |
dc.contributor.googleauthor | Choon Sang Bae | - |
dc.contributor.googleauthor | Byeong Jo Chun | - |
dc.contributor.googleauthor | Jeong Tae Koh | - |
dc.contributor.googleauthor | Seon Min Lee | - |
dc.identifier.doi | 10.1016/j.febslet.2004.03.106 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00446 | - |
dc.relation.journalcode | J00890 | - |
dc.identifier.eissn | 1873-3468 | - |
dc.identifier.pmid | 15147874 | - |
dc.subject.keyword | BAI1-AP4 | - |
dc.subject.keyword | brain-specific angiogenesis inhibitor 1-associated protein 4 | - |
dc.subject.keyword | HSV-TK | - |
dc.subject.keyword | herpes simplex virus-thymidine kinase | - |
dc.subject.keyword | NSE | - |
dc.subject.keyword | neuron-specific enolase | - |
dc.subject.keyword | TG | - |
dc.subject.keyword | transgenic | - |
dc.subject.keyword | BAI1-AP4 promoter | - |
dc.subject.keyword | Neuron-specific expression | - |
dc.subject.keyword | Transgene | - |
dc.subject.keyword | Developmental expression | - |
dc.contributor.alternativeName | Kim, Mi Young | - |
dc.contributor.affiliatedAuthor | Kim, Mi Young | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 566 | - |
dc.citation.number | 1-3 | - |
dc.citation.startPage | 87 | - |
dc.citation.endPage | 94 | - |
dc.identifier.bibliographicCitation | FEBS LETTERS, Vol.566(1-3) : 87-94, 2004 | - |
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