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MglA regulates transcription of virulence factors necessary for Francisella tularensis intraamoebae and intramacrophage survival

Authors
 Crystal M. Lauriano  ;  Jeffrey R. Barker  ;  Karl E. Klose  ;  Daniel J. Hassett  ;  Bernard P. Arulanandam  ;  Francis E. Nano  ;  Sang-Sun Yoon 
Citation
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.101(12) : 4246-4249, 2004 
Journal Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN
 0027-8424 
Issue Date
2004
MeSH
Amoeba/microbiology* ; Animals ; Bacterial Proteins/genetics* ; Bacterial Proteins/metabolism ; Female ; Francisellatularensis/genetics ; Francisellatularensis/metabolism ; Francisellatularensis/pathogenicity* ; Gene Expression Regulation* ; Host-Parasite Interactions ; Macrophages/microbiology* ; Mice ; Mutation
Abstract
Francisella tularensis is able to survive and grow within macrophages, a trait that contributes to pathogenesis. Several genes have been identified that are important for intramacrophage survival, including mglA and iglC. F. tularensis is also able to survive within amoebae. It is shown here that F. tularensis mglA and iglC mutant strains are not only defective for survival and replication within the macrophage-like cell line J774, but also within Acanthamoebae castellanii. Moreover, these strains are highly attenuated for virulence in mice, suggesting that a common mechanism underlies intramacrophage and intraamoebae survival and virulence. A 2D gel analysis of cell extracts of wild-type and mglA mutant strains revealed that at least seven prominent proteins were at low levels in the mglA mutant, and one MglA-regulated protein was identified as the IglC protein. RT-PCR analysis demonstrated reduced transcription of iglC and several other known and suspected virulence genes in the mglA mutant. Thus, MglA regulates the transcription of virulence factors of F. tularensis that contribute to intramacrophage and intraamoebae survival.
Files in This Item:
T200404390.pdf Download
DOI
10.1073/pnas.0307690101
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/112948
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