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Heterogeneity of the SR-dependent Inward Na+-Ca2+ Exchange Current in the Heavily Ca2+ - Buffered rat Ventricular Myocytes
DC Field | Value | Language |
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dc.contributor.author | 윤경봉 | - |
dc.date.accessioned | 2015-07-14T17:25:31Z | - |
dc.date.available | 2015-07-14T17:25:31Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112855 | - |
dc.description.abstract | Voltage-sensitive release mechanism was pharmacologically dissected from the Ca2+-induced Ca2+ release in the SR Ca2+ release in the rat ventricular myocytes patch-clamped in a whole-cell mode. SR Ca2+ release process was monitored by using forward-mode Na+-Ca2+ exchange after restriction of the interactions between Ca2+ from SR and Na+-Ca2+ exchange within micro-domains with heavy cytosolic Ca2+ buffering with 10 mM BAPTA. During stimulation every 10 s with a pulse roughly mimicking action potential, the initial outward current gradually turned into a huge inward current of -12.9+/-0.5 pA/pF. From the inward current, two different inward INCXs were identified. One was 10 muM ryanodine-sensitive, constituting 14.2+/-2.3%. It was completely blocked by CdCl2 (0.1 mM and 0.5 mM) and by Na+-depletion. The other was identified by 5 mM NiCl2 after suppression of ICaL and ryanodine receptor, constituting 14.8+/-1.6%. This latter was blocked by either 10 mM caffeine-induced SR Ca2+-depletion or 1 mM tetracaine. IV-relationships illustrated that the latter was activated until the peak in 30~35 mV lower voltages than the former. Overall, it was concluded that the SR Ca2+ release process in the rat ventricular myocytes is mediated by the voltage-sensitive release mechanism in addition to the Ca2+-induced-Ca2+ release. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 101~110 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Heterogeneity of the SR-dependent Inward Na+-Ca2+ Exchange Current in the Heavily Ca2+ - Buffered rat Ventricular Myocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anesthesiology (마취통증의학) | - |
dc.contributor.googleauthor | Kyung-Bong Yoon | - |
dc.contributor.googleauthor | Sung-Wan Ahn | - |
dc.contributor.googleauthor | Chang Mann Ko | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02539 | - |
dc.relation.journalcode | J02104 | - |
dc.identifier.eissn | 2093-3827 | - |
dc.subject.keyword | Voltage-sensitive release mechanism | - |
dc.subject.keyword | Ca2+-induced Ca2+ release | - |
dc.subject.keyword | Heavy Ca2+-buffering | - |
dc.subject.keyword | Na+-Ca2+ exchange | - |
dc.subject.keyword | SR | - |
dc.subject.keyword | Rat heart | - |
dc.contributor.alternativeName | Yoon, Kyoung Bong | - |
dc.contributor.affiliatedAuthor | Yoon, Kyoung Bong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 8 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 101 | - |
dc.citation.endPage | 110 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, Vol.8(2) : 101-110, 2004 | - |
dc.identifier.rimsid | 36756 | - |
dc.type.rims | ART | - |
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