Cited 89 times in
SREBP-1c mediates the insulin-dependent hepatic glucokinase expression
DC Field | Value | Language |
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dc.contributor.author | 김경섭 | - |
dc.date.accessioned | 2015-07-14T17:23:26Z | - |
dc.date.available | 2015-07-14T17:23:26Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112785 | - |
dc.description.abstract | The regulation of hepatic glucose metabolism is important in glucose homeostasis, and liver glucokinase (LGK) plays a central role in this process. Hepatic glucokinase expression is known to be regulated by insulin. Recently it has been suggested that sterol regulatory element binding protein-1c (SREBP-1c) mediates the action of insulin on LGK transcription; however, the precise mechanism is not, to date, well known. In the present study, we identified two functional SREBP-1c response elements, SREa and SREb, in the rat LGK promoter. SREBP-1c could bind to these SREs and activate the LGK promoter, and insulin activated the LGK promoter in Alexander cells. The physical interaction between the protein and SREs of the LGK promoter in vivo was also confirmed. Insulin selectively increased SREBP-1c and LGK expression in primary hepatocytes. Adenoviral expression of SREBP-1c stimulated LGK expression, and the dominant negative mutant of SREBP-1c blocked the increased gene expression of LGK by insulin and SREBP-1c. A chromatin immunoprecipitation assay using primary hepatocytes showed increased binding of SREBP-1 to SREs of the LGK promoter by insulin. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 30823~30829 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/genetics | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Blotting, Northern | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Proteins/metabolism | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Proteins/physiology* | - |
dc.subject.MESH | Carbohydrate Metabolism | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chromatin/metabolism | - |
dc.subject.MESH | DNA-Binding Proteins/metabolism | - |
dc.subject.MESH | DNA-Binding Proteins/physiology* | - |
dc.subject.MESH | Deoxyribonuclease I/metabolism | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Genes, Reporter | - |
dc.subject.MESH | Glucokinase/biosynthesis* | - |
dc.subject.MESH | Glucokinase/genetics* | - |
dc.subject.MESH | Glucokinase/metabolism | - |
dc.subject.MESH | Hepatocytes/metabolism | - |
dc.subject.MESH | Insulin/metabolism* | - |
dc.subject.MESH | Liver/enzymology* | - |
dc.subject.MESH | Luciferases/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Precipitin Tests | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | RNA/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Sterol Regulatory Element Binding Protein 1 | - |
dc.subject.MESH | Transcription Factors/metabolism | - |
dc.subject.MESH | Transcription, Genetic | - |
dc.subject.MESH | Transfection | - |
dc.title | SREBP-1c mediates the insulin-dependent hepatic glucokinase expression | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | So-Youn Kim | - |
dc.contributor.googleauthor | Ha-il Kim | - |
dc.contributor.googleauthor | Yong-Ho Ahn | - |
dc.contributor.googleauthor | Kyung-Sup Kim | - |
dc.contributor.googleauthor | In-Kyu Lee | - |
dc.contributor.googleauthor | Sang-Kyu Park | - |
dc.contributor.googleauthor | Seung-Soon Im | - |
dc.contributor.googleauthor | Tae-Hyun Kim | - |
dc.identifier.doi | 10.1074/jbc.M313223200 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00297 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 15123649 | - |
dc.contributor.alternativeName | Kim, Kyung Sup | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Sup | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 279 | - |
dc.citation.number | 29 | - |
dc.citation.startPage | 30823 | - |
dc.citation.endPage | 30829 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.279(29) : 30823-30829, 2004 | - |
dc.identifier.rimsid | 44504 | - |
dc.type.rims | ART | - |
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