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MN/CA9 촉진자를 가진 replication-competent 아데노바이러스를 이용한 신세포암에 대한 종양 특이적 유전자요법
DC Field | Value | Language |
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dc.contributor.author | 정현철 | - |
dc.date.accessioned | 2015-07-14T17:15:13Z | - |
dc.date.available | 2015-07-14T17:15:13Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0494-4747 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/112513 | - |
dc.description.abstract | Purpose: A new therapeutic approach is needed in patients with metastatic renal cell carcinoma (RCC) because of a dismal prognosis. MN/CA9 is a transmembrane glycoprotein that was first identified in the human cervical carcinoma cell line, HeLa. Since MN/CA9 protein is highly expressed in RCC tissues, but not in normal kidney, we constructed a tumor-specific replication-competent adenoviral vector utilizing MN/CA9 promoter (Ad-MN/CA9-E1a) and demonstrated its selective cytotoxicity toward MN/CA9-expressing RCC cells in vitro. Materials and Methods: MN/CA9-positive (HeLa, SK-RC-52) and MN/ CA9-negative (SK-RC-29) cells were used. RT-PCR assay for MN/CA9 mRNA was performed in each cells. Ad5 E1a protein production in each cells after infection with Ad-MN/CA9-E1a was determined by western blot analysis. In vitro cytotoxicity assay was performed for assessing the selective cytotoxicity of Ad-MN/CA9-E1a to MN/CA9-expressing cells. Results: RT-PCR assay showed that a distinct 255-bp fragment corresponding to the sequence within MN/CA9 cDNA was detected in HeLa and SK-RC-52 cells, but SK-RC-29 cells did not have MN/CA9 transcripts. Western blot analysis demonstrated that HeLa and SK-RC-52 cells showed much stronger Ad5 E1a protein expressions compared with SK-RC-29. In vitro cytotoxicity assay revealed that the growth of MN/CA9-positive cells was significantly inhibited with 0.1-1MOI of Ad-MN/CA9-E1a, but the growth of MN/CA9-negative cells (SK-RC-29) could only be inhibited by as many as 100MOI. Conclusions: These results suggest that a novel replication-competent adenoviral vector mediated by MN/CA9 promoter, Ad-MN/CA9-E1a, can selectively replicate in MN/CA9-expressing cancer cells with cytotoxic effects and may be utilized for the treatment of RCC. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 456~462 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF UROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | MN/CA9 촉진자를 가진 replication-competent 아데노바이러스를 이용한 신세포암에 대한 종양 특이적 유전자요법 | - |
dc.title.alternative | Tumor-specific Gene Therapy for Renal Cell Carcinoma Using MN/CA9-directed Replication-competent Adenovirus | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | 김세중 | - |
dc.contributor.googleauthor | 안미원 | - |
dc.contributor.googleauthor | 김영수 | - |
dc.contributor.googleauthor | 최민규 | - |
dc.contributor.googleauthor | 이상진 | - |
dc.contributor.googleauthor | 정현철 | - |
dc.contributor.googleauthor | 임호영 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J02135 | - |
dc.subject.keyword | Renal cell carcinoma | - |
dc.subject.keyword | Gene therapy | - |
dc.subject.keyword | Adenovirus | - |
dc.subject.keyword | Virus replication | - |
dc.contributor.alternativeName | Chung, Hyun Cheol | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 45 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 456 | - |
dc.citation.endPage | 462 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF UROLOGY, Vol.45(5) : 456-462, 2004 | - |
dc.identifier.rimsid | 56227 | - |
dc.type.rims | ART | - |
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