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저 파동성 전자기장이 인간 추간판 세포에 미치는 영향

Other Titles
 The Effect of Pulsed Electromagnetic Field in Human Intervertebral Disc Cell 
 권언혜  ;  문성환  ;  이환모  ;  김학선  ;  전지애  ;  이광일  ;  김향 
 Journal of Korean Society of Spine Surgery (대한척추외과학회지), Vol.11(4) : 194-201, 2004 
Journal Title
 Journal of Korean Society of Spine Surgery (대한척추외과학회지) 
Issue Date
Electromagnetic fields(EMF) ; Intervertebral disc(IVD) ; Proteoglycan
Study design An in vitro experiment. Objectives To assess the effect of pulsed sinusoidal EMF on human intervertebral disc (IVD) cells. Literature Review Summary Electromagnetic field (EMF) is known to modify some relevant physiological parameters of cells cultured in vitro, such as proliferation, synthesis, secretion of growth factors and transcription. EMF induces bone formation in delayed, non union and spinal fusion models. Also, the exposure of EMF has been shown to protect against the hazardous effect of smoking in the rabbit IVD. Materials and Methods Human IVD cells were three-dimensionally cultured in alginate beads and exposed to a 650Ω, 1.8millitesla magnetic flux density, 60Hz sinusoidal wave of EMF. The cultures were divided into the control and EMF groups, with various exposure times. The cytotoxicity, and DNA and proteoglycan syntheses were measured by the MTT assay, and [3H]-thymidine and [35S]-sulfate incorporation, respectively. RT-PCRs were performed for aggrecan, and collagen types I and II mRNA expressions. Results There was no recognizable cytotoxicity in the EMF group, but cellular proliferation was stimulated (p<0.05). Newly synthesized proteoglycan, normalized by DNA synthesis, was decreased in the EMF group (p<0.05) as were the expressions of aggrecan (48hour exposure) and type II collagen (72 hours exposure) mRNA compared to the control group. Conclusions EMF seems to be hazardous in the synthesis of the chondrogenic matrix, while marginally beneficial in the cellular proliferation of human IVD cells.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hak Sun(김학선) ORCID logo https://orcid.org/0000-0002-8330-4688
Moon, Seong Hwan(문성환)
Lee, Hwan Mo(이환모) ORCID logo https://orcid.org/0000-0002-5405-3832
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