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Smooth muscle-like tissues engineered with bone marrow stromal cells

Authors
 Seung-Woo Cho  ;  Il-Kwon Kim  ;  Byung-Soo Kim  ;  Cha Yong Choi  ;  Eun Yeol Lee  ;  Young Ha Kim  ;  Soo Hyun Kim  ;  Sun-Woong Kang  ;  Dong-Ik Kim  ;  Sang Hyun Lim 
Citation
 Biomaterials, Vol.25(15) : 2979-2986, 2004 
Journal Title
 Biomaterials 
ISSN
 0142-9612 
Issue Date
2004
Abstract
Bone marrow-derived cells have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. Here we tested whether smooth muscle (SM)-like tissues can be created in vivo with bone marrow stromal cells (BMSCs). Cultured canine BMSCs, which expressed SM cell-specific markers including SM α-actin and SM myosin heavy chain, were seeded on three-dimensional, biodegradable polymer scaffolds and implanted into peritoneal cavity of athymic mice. The cell–scaffold constructs retrieved 4 weeks after implantation formed three-dimensional tissues. Immunohistochemical analyses showed that the tissue reconstructs expressed SM α-actin and SM myosin heavy chain. Masson’s trichrome staining showed the presence of significant amounts of collagen in the tissue reconstructs. Cells labeled with a fluorescent tracer prior to implantation were still present in the tissue reconstructs 4 weeks after implantation. Non-seeded scaffolds (control groups) retrieved 4 weeks after implantation did not exhibit extensive tissue formation. This study demonstrates the potential of BMSCs as an alternative cell source for tissue engineering of SM.
Full Text
http://www.sciencedirect.com/science/article/pii/S014296120300810X
DOI
10.1016/j.biomaterials.2003.09.068
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
김일권(Kim, Il Kwon)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111618
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