Cited 140 times in
Diaphragmatic reconstruction with autologous tendon engineered from mesenchymal amniocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오정탁 | - |
dc.date.accessioned | 2015-07-14T16:47:23Z | - |
dc.date.available | 2015-07-14T16:47:23Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0022-3468 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111593 | - |
dc.description.abstract | PURPOSE: This study examined the effects of amniocyte-based engineered tendons on partial diaphragmatic replacement. METHODS: Ovine mesenchymal amniocytes were labeled with green fluorescent protein (GFP), expanded, and seeded into a collagen hydrogel. Composite grafts (20 to 25 cm2) based on acellular dermis (group I), or acellular small intestinal submucosa (group II) received either a cell-seeded or an acellular hydrogel within their layers. Newborn lambs (n = 20) underwent partial diaphragmatic replacement with either an acellular or a cellular autologous construct from either group. At 3 to 12 months' postoperatively, implants were subjected to multiple analyses. RESULTS: Diaphragmatic hernia recurrence was significantly higher in animals with acellular grafts (5 of 5) then in animals with cellular ones (1 of 4) in group I (P <.05) but not in group II (3 of 6 and 4 of 5, respectively). Cellular grafts had higher modular (5.27 +/- 1.98 v. 1.27 +/- 0.38 MPa) and ultimate (1.94 +/- 0.70 v. 0.29 +/- 0.05 MPa) tensile strength than acellular implants in group I (P <.05), but not in group II. Quantitative analyses showed no differences in extracellular matrix components between cellular and acellular implants in either group. All cellular implants showed GFP-positive cells. CONCLUSIONS: Diaphragmatic repair with an autologous tendon engineered from mesenchymal amniocytes leads to improved mechanical and functional outcomes when compared with an equivalent acellular bioprosthetic repair, depending on scaffold composition. The amniotic fluid may be a preferred cell source for engineered diaphragmatic reconstruction. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 834~838 | - |
dc.relation.isPartOf | JOURNAL OF PEDIATRIC SURGERY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amniotic Fluid/cytology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Animals, Newborn | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cells, Cultured/transplantation | - |
dc.subject.MESH | Collagen | - |
dc.subject.MESH | Collagen Type I/analysis | - |
dc.subject.MESH | Diaphragm/surgery* | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Elastin/analysis | - |
dc.subject.MESH | Genes, Reporter | - |
dc.subject.MESH | Glycosaminoglycans/analysis | - |
dc.subject.MESH | Hernia, Diaphragmatic/surgery | - |
dc.subject.MESH | Hernia, Diaphragmatic, Traumatic/surgery | - |
dc.subject.MESH | Hernias, Diaphragmatic, Congenital | - |
dc.subject.MESH | Hydrogels | - |
dc.subject.MESH | Laparotomy | - |
dc.subject.MESH | Mesenchymal Stem Cell Transplantation/methods* | - |
dc.subject.MESH | Mesenchymal Stem Cells/cytology* | - |
dc.subject.MESH | Sheep | - |
dc.subject.MESH | Tendons/transplantation* | - |
dc.subject.MESH | Tensile Strength | - |
dc.subject.MESH | Tissue Engineering* | - |
dc.subject.MESH | Transplantation, Autologous | - |
dc.title | Diaphragmatic reconstruction with autologous tendon engineered from mesenchymal amniocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | Julie R. Fuchs | - |
dc.contributor.googleauthor | Amir Kaviani | - |
dc.contributor.googleauthor | Jung-Tak Oh | - |
dc.contributor.googleauthor | David LaVan | - |
dc.contributor.googleauthor | Taturo Udagawa | - |
dc.contributor.googleauthor | Russell W. Jennings | - |
dc.contributor.googleauthor | Jay M. Wilson | - |
dc.contributor.googleauthor | Dario O. Fauza | - |
dc.identifier.doi | 10.1016/j.jpedsurg.2004.02.014 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J01689 | - |
dc.identifier.eissn | 1531-5037 | - |
dc.identifier.pmid | 15185207 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0022346804000971 | - |
dc.subject.keyword | Congenital diaphragmatic hernia | - |
dc.subject.keyword | tissue engineering | - |
dc.subject.keyword | amniotic fluid | - |
dc.subject.keyword | diaphragm | - |
dc.subject.keyword | congenital anomalies | - |
dc.subject.keyword | birth defects | - |
dc.subject.keyword | fetus | - |
dc.subject.keyword | prenatal | - |
dc.subject.keyword | neonate | - |
dc.subject.keyword | transplantation | - |
dc.contributor.alternativeName | Oh, Jung Tak | - |
dc.contributor.affiliatedAuthor | 오정탁 | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 39 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 834 | - |
dc.citation.endPage | 838 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PEDIATRIC SURGERY, Vol.39(6) : 834-838, 2004 | - |
dc.identifier.rimsid | 37351 | - |
dc.type.rims | ART | - |
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