Cited 73 times in
Extracellular fragment of brain-specific angiogenesis inhibitor 1 suppresses endothelial cell proliferation by blocking αvβ5 integrin
DC Field | Value | Language |
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dc.contributor.author | 김미영 | - |
dc.date.accessioned | 2015-07-14T16:46:43Z | - |
dc.date.available | 2015-07-14T16:46:43Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0014-4827 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111570 | - |
dc.description.abstract | Brain-specific angiogenesis inhibitor 1 (BAI1) is a transmembrane protein with anti-angiogenic activity. The mechanisms underlying BAI1 activity are unknown. In this study, we found that overexpression of BAI1 increased cell death in human umbilical vein endothelial cells (HUVECs) and, to a lesser degree, in SHSY5Y and U343 cells. Conditioned medium from BAI1-transfected U343 cells inhibited proliferation of HUVECs, and this effect was neutralized by addition of anti-BAI1 serum. The conditioned medium contained four cleavage products of the BAI1 extracellular domain. BAI1's middle extracellular region containing five thrombospondin type 1 repeats (BAI1-TSR) was sufficient for BAI1's antiproliferative effect on HUVECs. BAI1's action on HUVECs was blocked by anti-αv integrin, but not by anti-CD36 antibody treatment. Introduction of αvβ5 integrin into HEK293 cells rendered them susceptible to cell death by BAI1, and BAI1-TSR bound with αvβ5 integrin, but not to αvβ3 integrin in brain tissue. Fluorescent BAI1-TSR colocalized with αvβ5 integrin in HUVECs. Together, our results indicate that BAI1 has antiproliferative action on surrounding endothelial cells by blocking αvβ5 integrin, and its active region is BAI1-TSR. BAI1-TSR could be valuable for regulating brain angiogenesis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 172~184 | - |
dc.relation.isPartOf | EXPERIMENTAL CELL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Angiogenesis Inhibitors/chemistry | - |
dc.subject.MESH | Angiogenesis Inhibitors/metabolism | - |
dc.subject.MESH | Angiogenesis Inhibitors/pharmacology* | - |
dc.subject.MESH | Angiogenic Proteins/chemistry | - |
dc.subject.MESH | Angiogenic Proteins/metabolism | - |
dc.subject.MESH | Angiogenic Proteins/pharmacology* | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Brain/metabolism | - |
dc.subject.MESH | CD36 Antigens/immunology | - |
dc.subject.MESH | Caspase Inhibitors | - |
dc.subject.MESH | Cell Division/drug effects | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Culture Media, Conditioned | - |
dc.subject.MESH | Cysteine Proteinase Inhibitors/pharmacology | - |
dc.subject.MESH | Endothelium, Vascular/cytology | - |
dc.subject.MESH | Endothelium, Vascular/drug effects* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Integrin alphaV/immunology | - |
dc.subject.MESH | Integrins/analysis | - |
dc.subject.MESH | Integrins/antagonists & inhibitors* | - |
dc.subject.MESH | Integrins/physiology | - |
dc.subject.MESH | Necrosis | - |
dc.subject.MESH | Peptide Fragments/pharmacology | - |
dc.subject.MESH | Receptors, Vitronectin/analysis | - |
dc.subject.MESH | Receptors, Vitronectin/antagonists & inhibitors* | - |
dc.subject.MESH | Receptors, Vitronectin/physiology | - |
dc.title | Extracellular fragment of brain-specific angiogenesis inhibitor 1 suppresses endothelial cell proliferation by blocking αvβ5 integrin | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Jeong Tae Koh | - |
dc.contributor.googleauthor | Hyun Kook | - |
dc.contributor.googleauthor | Kyung Keun Kim | - |
dc.contributor.googleauthor | Shin Jung | - |
dc.contributor.googleauthor | Kyung Chul Yoon | - |
dc.contributor.googleauthor | Mi-Young Kim | - |
dc.contributor.googleauthor | Ji Hee Lee | - |
dc.contributor.googleauthor | Byung Chul Jeong | - |
dc.contributor.googleauthor | Young-Woo Seo | - |
dc.contributor.googleauthor | Hae Jin Kee | - |
dc.identifier.doi | 10.1016/j.yexcr.2003.11.008 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J00865 | - |
dc.identifier.eissn | 1090-2422 | - |
dc.identifier.pmid | 14980512 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0014482703006177 | - |
dc.subject.keyword | Brain-specific angiogenesis inhibitor 1 (BAI1) | - |
dc.subject.keyword | Neuron-specific gene | - |
dc.subject.keyword | Endothelial cells | - |
dc.subject.keyword | αvβ5 integrin | - |
dc.subject.keyword | Antiproliferative action | - |
dc.contributor.alternativeName | Kim, Mi Young | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 294 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 172 | - |
dc.citation.endPage | 184 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL CELL RESEARCH, Vol.294(1) : 172-184, 2004 | - |
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