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Extracellular fragment of brain-specific angiogenesis inhibitor 1 suppresses endothelial cell proliferation by blocking αvβ5 integrin

DC FieldValueLanguage
dc.contributor.author김미영-
dc.date.accessioned2015-07-14T16:46:43Z-
dc.date.available2015-07-14T16:46:43Z-
dc.date.issued2004-
dc.identifier.issn0014-4827-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111570-
dc.description.abstractBrain-specific angiogenesis inhibitor 1 (BAI1) is a transmembrane protein with anti-angiogenic activity. The mechanisms underlying BAI1 activity are unknown. In this study, we found that overexpression of BAI1 increased cell death in human umbilical vein endothelial cells (HUVECs) and, to a lesser degree, in SHSY5Y and U343 cells. Conditioned medium from BAI1-transfected U343 cells inhibited proliferation of HUVECs, and this effect was neutralized by addition of anti-BAI1 serum. The conditioned medium contained four cleavage products of the BAI1 extracellular domain. BAI1's middle extracellular region containing five thrombospondin type 1 repeats (BAI1-TSR) was sufficient for BAI1's antiproliferative effect on HUVECs. BAI1's action on HUVECs was blocked by anti-αv integrin, but not by anti-CD36 antibody treatment. Introduction of αvβ5 integrin into HEK293 cells rendered them susceptible to cell death by BAI1, and BAI1-TSR bound with αvβ5 integrin, but not to αvβ3 integrin in brain tissue. Fluorescent BAI1-TSR colocalized with αvβ5 integrin in HUVECs. Together, our results indicate that BAI1 has antiproliferative action on surrounding endothelial cells by blocking αvβ5 integrin, and its active region is BAI1-TSR. BAI1-TSR could be valuable for regulating brain angiogenesis.-
dc.description.statementOfResponsibilityopen-
dc.format.extent172~184-
dc.relation.isPartOfEXPERIMENTAL CELL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAngiogenesis Inhibitors/chemistry-
dc.subject.MESHAngiogenesis Inhibitors/metabolism-
dc.subject.MESHAngiogenesis Inhibitors/pharmacology*-
dc.subject.MESHAngiogenic Proteins/chemistry-
dc.subject.MESHAngiogenic Proteins/metabolism-
dc.subject.MESHAngiogenic Proteins/pharmacology*-
dc.subject.MESHApoptosis-
dc.subject.MESHBrain/metabolism-
dc.subject.MESHCD36 Antigens/immunology-
dc.subject.MESHCaspase Inhibitors-
dc.subject.MESHCell Division/drug effects-
dc.subject.MESHCell Line-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCulture Media, Conditioned-
dc.subject.MESHCysteine Proteinase Inhibitors/pharmacology-
dc.subject.MESHEndothelium, Vascular/cytology-
dc.subject.MESHEndothelium, Vascular/drug effects*-
dc.subject.MESHHumans-
dc.subject.MESHIntegrin alphaV/immunology-
dc.subject.MESHIntegrins/analysis-
dc.subject.MESHIntegrins/antagonists & inhibitors*-
dc.subject.MESHIntegrins/physiology-
dc.subject.MESHNecrosis-
dc.subject.MESHPeptide Fragments/pharmacology-
dc.subject.MESHReceptors, Vitronectin/analysis-
dc.subject.MESHReceptors, Vitronectin/antagonists & inhibitors*-
dc.subject.MESHReceptors, Vitronectin/physiology-
dc.titleExtracellular fragment of brain-specific angiogenesis inhibitor 1 suppresses endothelial cell proliferation by blocking αvβ5 integrin-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Biochemistry & Molecular Biology (생화학,분자생물학)-
dc.contributor.googleauthorJeong Tae Koh-
dc.contributor.googleauthorHyun Kook-
dc.contributor.googleauthorKyung Keun Kim-
dc.contributor.googleauthorShin Jung-
dc.contributor.googleauthorKyung Chul Yoon-
dc.contributor.googleauthorMi-Young Kim-
dc.contributor.googleauthorJi Hee Lee-
dc.contributor.googleauthorByung Chul Jeong-
dc.contributor.googleauthorYoung-Woo Seo-
dc.contributor.googleauthorHae Jin Kee-
dc.identifier.doi10.1016/j.yexcr.2003.11.008-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.relation.journalcodeJ00865-
dc.identifier.eissn1090-2422-
dc.identifier.pmid14980512-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0014482703006177-
dc.subject.keywordBrain-specific angiogenesis inhibitor 1 (BAI1)-
dc.subject.keywordNeuron-specific gene-
dc.subject.keywordEndothelial cells-
dc.subject.keywordαvβ5 integrin-
dc.subject.keywordAntiproliferative action-
dc.contributor.alternativeNameKim, Mi Young-
dc.rights.accessRightsnot free-
dc.citation.volume294-
dc.citation.number1-
dc.citation.startPage172-
dc.citation.endPage184-
dc.identifier.bibliographicCitationEXPERIMENTAL CELL RESEARCH, Vol.294(1) : 172-184, 2004-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers

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