Cited 10 times in
Targeting of therapeutic gene expression to the liver by using liver-type pyruvate kinase proximal promoter and the SV40 viral enhancer active in multiple cell types
DC Field | Value | Language |
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dc.contributor.author | 안용호 | - |
dc.contributor.author | 안철우 | - |
dc.contributor.author | 이현철 | - |
dc.contributor.author | 김경섭 | - |
dc.date.accessioned | 2015-07-14T16:46:00Z | - |
dc.date.available | 2015-07-14T16:46:00Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111546 | - |
dc.description.abstract | To achieve the liver-directed expression in sufficient amounts of therapeutic genes for successful and safe gene therapy, natural liver-specific promoters can be used to direct the expression of therapeutic genes in the liver, whereas strong viral enhancers were used to obtain sufficient amounts of expressed therapeutic gene products. However, very often use of either the former or the latter does not guarantee both potent and liver-specific therapeutic gene expression. Here we conglomerate them and thus create a potent tissue-specific promoter by characterizing and using the liver-type pyruvate kinase proximal promoter (LPKPP) harboring its TATA box and a HNF-1α binding site. Alone it hardly activated its reporter gene expression in non-hepatocytes or hepatocytes. However, in the presence of the SV40 viral enhancer (SV40VE), which is active in multiple cell types, it was able to potently activate its reporter gene expression specifically in hepatocytes. The tissue-specific activation of the LPKPP by the viral enhancer was attributed to HNF-1α binding to the LPKPP. Taken together, these results support the idea that the constitutively active SV40VE could be used to activate the LPKPP in a tissue-specific manner in the presence of HNF-1α. To our knowledge, this is the first study to utilize HNF-1α and its binding site, in the context of the LPKPP, to generate a basal promoter that is transcriptionally activated potently in a tissue-specific manner by a viral enhancer that is active in multiple cell types. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 131~137 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | DNA-Binding Proteins/genetics* | - |
dc.subject.MESH | DNA-Binding Proteins/metabolism* | - |
dc.subject.MESH | Enhancer Elements, Genetic/genetics | - |
dc.subject.MESH | Gene Expression Regulation/genetics | - |
dc.subject.MESH | Gene Targeting/methods* | - |
dc.subject.MESH | Genetic Therapy/methods | - |
dc.subject.MESH | Hepatoblastoma/genetics | - |
dc.subject.MESH | Hepatoblastoma/metabolism | - |
dc.subject.MESH | Hepatocyte Nuclear Factor 1 | - |
dc.subject.MESH | Hepatocyte Nuclear Factor 1-alpha | - |
dc.subject.MESH | Hepatocyte Nuclear Factor 1-beta | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kidney/metabolism | - |
dc.subject.MESH | Liver/metabolism* | - |
dc.subject.MESH | Nuclear Proteins* | - |
dc.subject.MESH | Organ Specificity | - |
dc.subject.MESH | Promoter Regions, Genetic/genetics | - |
dc.subject.MESH | Pyruvate Kinase/genetics* | - |
dc.subject.MESH | Pyruvate Kinase/metabolism* | - |
dc.subject.MESH | Transcription Factors/genetics* | - |
dc.subject.MESH | Transcription Factors/metabolism* | - |
dc.title | Targeting of therapeutic gene expression to the liver by using liver-type pyruvate kinase proximal promoter and the SV40 viral enhancer active in multiple cell types | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Cheol Won Park | - |
dc.contributor.googleauthor | Young Mi Park | - |
dc.contributor.googleauthor | Hyun Chul Lee | - |
dc.contributor.googleauthor | Yong-ho Ahn | - |
dc.contributor.googleauthor | Kyung-Sup Kim | - |
dc.contributor.googleauthor | Bong Soo Cha | - |
dc.contributor.googleauthor | Chul Woo Ahn | - |
dc.contributor.googleauthor | Ji-Young Cha | - |
dc.contributor.googleauthor | Seonock Woo | - |
dc.contributor.googleauthor | Yongho Lee | - |
dc.contributor.googleauthor | Geun Taek Lee | - |
dc.identifier.doi | 10.1016/j.bbrc.2003.12.064 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02989 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 14715256 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X03026585 | - |
dc.subject.keyword | Gene therapy | - |
dc.subject.keyword | L-PK proximal promoter | - |
dc.subject.keyword | HNF-1α | - |
dc.subject.keyword | SV40 viral enhancer | - |
dc.subject.keyword | Tissue-specific gene expression | - |
dc.contributor.alternativeName | Ahn, Yong Ho | - |
dc.contributor.alternativeName | Ahn, Chul Woo | - |
dc.contributor.alternativeName | Lee, Hyun Chul | - |
dc.contributor.alternativeName | Kim, Kyung Sup | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 314 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 131 | - |
dc.citation.endPage | 137 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.314(1) : 131-137, 2004 | - |
dc.identifier.rimsid | 34910 | - |
dc.type.rims | ART | - |
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