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MMP expression profiling in recurred stage IB lung cancer

DC Field Value Language
dc.contributor.author강숙희-
dc.contributor.author정경영-
dc.contributor.author조남훈-
dc.contributor.author조상호-
dc.date.accessioned2015-07-14T16:45:25Z-
dc.date.available2015-07-14T16:45:25Z-
dc.date.issued2004-
dc.identifier.issn0950-9232-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111527-
dc.description.abstractWe aimed to clarify the prime role of recurrence in stage I lung cancer. To determine the expression profiles, quantitative RT–PCR and real-time PCR were performed subsequently to evaluate the validity of meaningful molecules identified by 0.12 K c-DNA array experiment surveys. In all, 10 lung cancer patients presenting with recurrence of stage IB were selected and compared with 10 stage IB lung cancer patients without recurrence since biopsied 3 years previously. On c-DNA microarray data analysis using pairs of recurred and the corresponding nonrecurred patients, the following genes were found to be upregulated in the recurred cases: matrix metalloproteinase (MMP)-10 in five cases, MMP-12 in two cases, MMP-11, MMP-14, MMP-15, fos, cyclin E2, E2F3, TGF-alpha in each one case. The most frequently upregulated genes in recurred lung cancers were MMP-10 (stromelysin-2) and MMP-12 (macrophage elastase). On transcriptional assay by quantitative RT–PCR and real-time RT–PCR analysis to validate those molecules, both transcripts of MMP-10 and MMP-12 were significantly more upregulated in recurred stage IB lung cancer than in the non-recurred stage IB lung cancer (P=0.004). Transcript levels were identical to c-DNA array data. The protein levels of these entities were also evaluated by immunohistochemistry of archival slides. By immunohistochemistry, MMP-10 monoclonal antibody showed more intense immunoreactivity in the recurred stage IB lung cancer than in the nonrecurred stage IB lung cancer (P=0.0313). Our approach revealed that MMP-10 plays an important role in the recurrence in stage IB lung cancer, irrespective of the histologic type.-
dc.description.statementOfResponsibilityopen-
dc.format.extent845~851-
dc.relation.isPartOfONCOGENE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBase Sequence-
dc.subject.MESHCarcinoma, Non-Small-CellLung/enzymology-
dc.subject.MESHCarcinoma, Non-Small-CellLung/genetics*-
dc.subject.MESHDNA Primers-
dc.subject.MESHGeneExpressionProfiling*-
dc.subject.MESHHumans-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHLungNeoplasms/enzymology-
dc.subject.MESHLungNeoplasms/genetics*-
dc.subject.MESHMatrix Metalloproteinases/genetics*-
dc.subject.MESHOligonucleotide Array Sequence Analysis-
dc.subject.MESHRecurrence-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHTranscription, Genetic-
dc.titleMMP expression profiling in recurred stage IB lung cancer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorNam Hoon Cho-
dc.contributor.googleauthorKyi Pyo Hong-
dc.contributor.googleauthorSang Ho Cho-
dc.contributor.googleauthorKyung Young Chung-
dc.contributor.googleauthorSuki Kang-
dc.contributor.googleauthorSung Hui Hong-
dc.identifier.doi10.1038/sj.onc.1207140-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.relation.journalcodeJ02413-
dc.identifier.eissn1476-5594-
dc.identifier.pmid14647437-
dc.identifier.urlhttp://www.nature.com/onc/journal/v23/n3/full/1207140a.html-
dc.contributor.alternativeNameKang, Suki-
dc.contributor.alternativeNameChung, Kyung Young-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.alternativeNameCho, Sang Ho-
dc.rights.accessRightsnot free-
dc.citation.volume23-
dc.citation.number3-
dc.citation.startPage845-
dc.citation.endPage851-
dc.identifier.bibliographicCitationONCOGENE, Vol.23(3) : 845-851, 2004-
dc.identifier.rimsid34897-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers

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