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German Cockroach Extract Induces Activation of Human Eosinophils to Release Cytotoxic Inflammatory Mediators

Authors
 Sohn M.-H.  ;  Lee Y.-A.  ;  Shin M.H.  ;  Yong T.-S.  ;  Kim K.-E.  ;  Sim S.  ;  Jeong K.-Y. 
Citation
 INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.134(2) : 141-149, 2004 
Journal Title
 INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY 
ISSN
 1018-2438 
Issue Date
2004
MeSH
Allergens/administration & dosage ; Allergens/pharmacology* ; Animals ; Antigens, Differentiation/biosynthesis ; Antigens, Differentiation/drug effects ; Cell Degranulation/drug effects ; Cockroaches* ; Cytotoxicity, Immunologic/drug effects ; Dose-Response Relationship, Drug ; Enzyme Activation/drug effects ; Eosinophil-Derived Neurotoxin ; Eosinophils/drug effects* ; Eosinophils/metabolism ; Eosinophils/physiology* ; Exocytosis/drug effects ; Humans ; Inflammation Mediators/metabolism* ; Interleukin-5/administration & dosage ; Mitogen-Activated Protein Kinases/drug effects ; Mitogen-Activated Protein Kinases/metabolism ; Phosphorylation/drug effects ; Ribonucleases/drug effects ; Ribonucleases/metabolism ; Signal Transduction/drug effects ; Superoxides/metabolism ; p38 Mitogen-Activated Protein Kinases
Abstract
BACKGROUND: Eosinophils play an important role in the pathogenesis of allergic diseases. Sensitization and exposure to cockroach allergen have been demonstrated to be one of the major risk factors for the development of bronchial asthma. However, little is known regarding the functional capacity of cockroach extract antigen to activate human eosinophils. OBJECTIVE: We investigated whether German cockroach extract can activate human eosinophils to release cytotoxic inflammatory mediators. METHODS: Purified eosinophils from the peripheral blood were incubated with various concentrations (0-200 microg/ml) of German cockroach extract antigen. Effector functions of eosinophils were checked by degranulation and superoxide anion production. In addition, we examined surface expression of CD11b and CD69, and intracellular activation of p38 mitogen-activated protein kinase (p38 MAP kinase) in cockroach-stimulated eosinophils. RESULTS: German cockroach extract induced degranulation and superoxide production from human eosinophils. In addition, incubation of eosinophils for 3 h with the cockroach extract resulted in an increased level of the surface expression of CD11b and CD69. Furthermore, cockroach-induced superoxide production from eosinophils was significantly inhibited by the pretreatment of cells with a p38 MAP kinase inhibitor SB202190. Indeed, a large amount of phosphorylated forms of p38 MAP kinase was detected in cockroach-stimulated eosinophils. CONCLUSIONS: Our results suggest that German cockroach extract induces activation of human eosinophils to release cytotoxic inflammatory mediators such as superoxide and granular proteins.
Full Text
http://www.karger.com/Article/FullText/78647
DOI
10.1159/000078647
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Earn(김규언)
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Lee, Young Ah(이영아) ORCID logo https://orcid.org/0000-0002-0414-842X
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111515
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