Cited 19 times in
Investigation of extended-spectrum β-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배일권 | - |
dc.contributor.author | 정석훈 | - |
dc.date.accessioned | 2015-07-14T16:41:11Z | - |
dc.date.available | 2015-07-14T16:41:11Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0266-8254 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111385 | - |
dc.description.abstract | AIMS: Isolates obtained from various regions in Korea in 2002 were identified and their susceptibility to extended-spectrum cephalosporins, monobactams and/or cephamycins was studied along with any production of extended-spectrum beta-lactamases (ESBLs). METHODS AND RESULTS: Bacteria identified by the conventional techniques and Vitek GNI card were Klebsiella pneumoniae and Escherichia coli. Using disk diffusion and double-disk synergy tests, we found that 39.2% of strains produced ESBLs. About 52% of isolates transferred resistance to ceftazidime by conjugation. Banding patterns of PCR amplification with the designed primers showed that 837- and 259-bp fragments specific to bla(TEM) genes were amplified in 63.3% of strains. 929- and 231-bp fragments (bla(SHV)), 847- and 520-bp fragments (bla(CMY)), 597- and 858-bp fragments (bla(CTX-M)) were amplified in 61.5, 17.3 and 7.7% of strains respectively. About 51.9% of strains contained more than two types of beta-lactamase genes. Especially, one strain contained bla(TEM), bla(CMY) and bla(CTX-M) genes. SIGNIFICANCE: Resistance mechanisms to beta-lactams, comprising mostly ESBL production, lead to the resistance against even recently developed beta-lactams in enterobacteria, which is now a serious threat to antibiotic therapy. The high prevalence of bla(CMY) genes and multidrug-resistant genes may also make therapeutic failure and lack of eradiation of these strains by extended-spectrum cephalosporins or cephamycins. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 41~47 | - |
dc.relation.isPartOf | LETTERS IN APPLIED MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anti-Bacterial Agents/pharmacology* | - |
dc.subject.MESH | Cephalosporins/pharmacology | - |
dc.subject.MESH | Drug Resistance, Bacterial* | - |
dc.subject.MESH | Escherichia coli/classification | - |
dc.subject.MESH | Escherichia coli/drug effects | - |
dc.subject.MESH | Escherichia coli/enzymology* | - |
dc.subject.MESH | Escherichia coli/genetics | - |
dc.subject.MESH | Escherichia coli Infections/epidemiology | - |
dc.subject.MESH | Escherichia coli Infections/microbiology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Klebsiella Infections/epidemiology | - |
dc.subject.MESH | Klebsiella Infections/microbiology | - |
dc.subject.MESH | Klebsiella pneumoniae/classification | - |
dc.subject.MESH | Klebsiella pneumoniae/drug effects | - |
dc.subject.MESH | Klebsiella pneumoniae/enzymology* | - |
dc.subject.MESH | Klebsiella pneumoniae/genetics | - |
dc.subject.MESH | Korea/epidemiology | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | beta-Lactamases/metabolism* | - |
dc.subject.MESH | beta-Lactams/pharmacology* | - |
dc.title | Investigation of extended-spectrum β-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | S.H. Jeong | - |
dc.contributor.googleauthor | I.K. Bae | - |
dc.contributor.googleauthor | S.H. Lee | - |
dc.contributor.googleauthor | S.-J. Kim | - |
dc.contributor.googleauthor | B.C. Jeong | - |
dc.contributor.googleauthor | J.S. Song | - |
dc.contributor.googleauthor | H.I. Jung | - |
dc.contributor.googleauthor | J.H. Lee | - |
dc.contributor.googleauthor | S.B. Kwon | - |
dc.identifier.doi | 10.1111/j.1472-765X.2004.01537.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J02163 | - |
dc.identifier.eissn | 1472-765X | - |
dc.identifier.pmid | 15189286 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2004.01537.x/abstract | - |
dc.subject.keyword | cephamycins | - |
dc.subject.keyword | CMY | - |
dc.subject.keyword | CTX‐M | - |
dc.subject.keyword | extended‐spectrum cephalosporins | - |
dc.subject.keyword | SHV | - |
dc.subject.keyword | TEM | - |
dc.contributor.alternativeName | Bae, Il Kwon | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 39 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 41 | - |
dc.citation.endPage | 47 | - |
dc.identifier.bibliographicCitation | LETTERS IN APPLIED MICROBIOLOGY, Vol.39(1) : 41-47, 2004 | - |
dc.identifier.rimsid | 35783 | - |
dc.type.rims | ART | - |
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