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Proliferation, differentiation, and calcification of preosteoblast-like MC3T3-E1 cells cultured onto noncrystalline calcium phosphate glass

Authors
 Yong-Keun Lee  ;  Jin Song  ;  Kyoung-Nam Kim  ;  Racquel Z. LeGeros  ;  Chong-Kwan Kim  ;  Seong-Ho Choi  ;  Kwang-Mahn Kim  ;  Sang-Bae Lee 
Citation
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Vol.69A(1) : 188-195, 2004 
Journal Title
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
ISSN
 1549-3296 
Issue Date
2004
Keywords
bond graft ; calcium phosphate ; MC3T3‐E1 cell ; proliferation ; differentiation ; calcification
Abstract
The purpose of this study was to investigate calcium phosphate glass as a potential biomaterial for hard tissue repair. We prepared calcium phosphate glass using the system CaO-CaF(2)-P(2)O(5)-MgO-ZnO and cultured MC3T3-E1 cells onto the glass in alpha-MEM with beta-glycerophosphatase and ascorbic acid. Proliferation of the cells was determined to evaluate the biocompatibility of the prepared calcium phosphate glass. The alkaline phosphatase activity was measured to examine the osteoblast differentiation. Mineralization was evaluated by staining the calcium precipitates with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant influence on cell proliferation compared to the polystyrene chosen as a control in this experiment (p > 0.05). The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days after incubation than that of the control group (p < 0.05). The promotion of bone-like tissue formation by the calcium phosphate glass was observed after 7 days and thereafter. The results of the present study indicate that the prepared calcium phosphate glass affects osteogenesis by increasing calcification of the extracellular matrix.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.20137/abstract
DOI
10.1002/jbm.a.20137
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Kim, Chong Kwan(김종관)
Song, Jin(송진)
Lee, Yong Keun(이용근)
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
Choi, Seung Ho(최승호) ORCID logo https://orcid.org/0000-0002-9872-3594
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111303
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