Cited 20 times in
Expression and cross-species reactivity of fatty acid-binding protein of Clonorchis sinensis
DC Field | Value | Language |
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dc.contributor.author | 용태순 | - |
dc.date.accessioned | 2015-07-14T16:37:34Z | - |
dc.date.available | 2015-07-14T16:37:34Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0932-0113 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111265 | - |
dc.description.abstract | Clonorchis sinensis is a Chinese liver fluke that chronically resides in the biliary tract. The fatty acid-binding protein (FABP) is known to play an important role in the intracellular transport of long-chain fatty acids that are obtained by the fluke from the host. Although FABP has stimulated considerable interest as a vaccine target candidate, the nature of FABP from C. sinensis (CsFABP) remains unclear. In this paper, we describe the cloning and expression of recombinant FABP and immune cross-reaction by Western blot analysis. Sequence analysis revealed that the CsFABP cDNA contained a single open reading frame (ORF) coding for 134 amino acids with an estimated molecular mass of a 15.2 kDa. The DNA sequence of CsFABP cDNA showed significant homology to schistosome cytosolic FABPs, with a 49% amino acid sequence identity and 89% similarity to Schistosoma japonicum. This DNA also showed a high sequence similarity at the amino acid level to S. mansoni (Sm14; 83%) and Fasciola hepatica (80%). The CsFABP cDNA was cloned into expression vector pET28a, expressed in Escherichia coli and the recombinant protein purified by affinity chromatography. The recombinant CsFABP was cross-reacted with sera obtained from patients with fascioliasis and paragonimiasis. These results suggest that CsFABP may be useful as a vaccine for clonorchiasis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 339~343 | - |
dc.relation.isPartOf | PARASITOLOGY RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Helminth/blood | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Carrier Proteins/chemistry | - |
dc.subject.MESH | Carrier Proteins/genetics* | - |
dc.subject.MESH | Carrier Proteins/immunology* | - |
dc.subject.MESH | Carrier Proteins/metabolism | - |
dc.subject.MESH | Cloning, Molecular | - |
dc.subject.MESH | Clonorchis sinensis/genetics* | - |
dc.subject.MESH | Clonorchis sinensis/metabolism* | - |
dc.subject.MESH | Cross Reactions | - |
dc.subject.MESH | DNA, Complementary | - |
dc.subject.MESH | DNA, Helminth/chemistry | - |
dc.subject.MESH | Escherichia coli/genetics | - |
dc.subject.MESH | Escherichia coli/metabolism | - |
dc.subject.MESH | Fasciola hepatica/genetics | - |
dc.subject.MESH | Fascioliasis/immunology | - |
dc.subject.MESH | Fascioliasis/parasitology | - |
dc.subject.MESH | Fatty Acid-Binding Proteins | - |
dc.subject.MESH | Helminth Proteins/chemistry | - |
dc.subject.MESH | Helminth Proteins/genetics* | - |
dc.subject.MESH | Helminth Proteins/immunology | - |
dc.subject.MESH | Helminth Proteins/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Molecular Weight | - |
dc.subject.MESH | Open Reading Frames | - |
dc.subject.MESH | Paragonimiasis/immunology | - |
dc.subject.MESH | Paragonimiasis/parasitology | - |
dc.subject.MESH | Recombinant Proteins/chemistry | - |
dc.subject.MESH | Recombinant Proteins/immunology | - |
dc.subject.MESH | Recombinant Proteins/metabolism | - |
dc.subject.MESH | Schistosoma japonicum/genetics | - |
dc.subject.MESH | Schistosoma mansoni/genetics | - |
dc.subject.MESH | Sequence Alignment | - |
dc.subject.MESH | Sequence Analysis, DNA | - |
dc.subject.MESH | Sequence Homology, Amino Acid | - |
dc.title | Expression and cross-species reactivity of fatty acid-binding protein of Clonorchis sinensis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Ji-Sook Lee Tai-Soon Yong | - |
dc.identifier.doi | 10.1007/s00436-004-1139-z | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J02467 | - |
dc.identifier.eissn | 1432-1955 | - |
dc.identifier.pmid | 15197581 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs00436-004-1139-z | - |
dc.subject.keyword | Adult Worm | - |
dc.subject.keyword | Schistosoma Mansoni | - |
dc.subject.keyword | Schistosoma Japonicum | - |
dc.subject.keyword | Fascioliasis | - |
dc.subject.keyword | Clonorchiasis | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 93 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 339 | - |
dc.citation.endPage | 343 | - |
dc.identifier.bibliographicCitation | PARASITOLOGY RESEARCH, Vol.93(5) : 339-343, 2004 | - |
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