Cited 31 times in
Differential role of the loop region between helices H6 and H7 within the orphan nuclear receptors small heterodimer partner and DAX-1
DC Field | Value | Language |
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dc.contributor.author | 김미영 | - |
dc.date.accessioned | 2015-07-14T16:34:41Z | - |
dc.date.available | 2015-07-14T16:34:41Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0888-8809 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111173 | - |
dc.description.abstract | The orphan nuclear receptors small heterodimer partner (SHP) and dosage-sensitive sex-reversal adrenal hypoplasia congenital (AHC) critical region on the X chromosome gene 1 (DAX-1) contain extra amino acids between helices H6 and H7 of LBD, and here we investigated a possible role of these additional amino acids. Transient transfection assay demonstrated that, in contrast to wild type, in mutant SHP Delta128-139 deletion of 12 extra amino acids in H6-H7 failed to repress the transactivity of orphan nuclear receptors such as estrogen receptor-related receptor-gamma, hepatocyte nuclear factor 4alpha, and constitutive androstane receptor. Interestingly, yeast two-hybrid and glutathione-S-transferase pull-down assays demonstrated that wild-type and SHP Delta128-139 have similar abilities to interact with estrogen receptor-related receptor-gamma, hepatocyte nuclear factor 4alpha, and constitutive androstane receptor. Unexpectedly, in wild-type DAX-1 and mutant DAX-1 Delta338-362, deletion of 25 extra amino acids in H6-H7 had no significant difference in the interaction and repression of steroidogenic factor 1 transactivation. Mutant SHP that contains DAX-1 extra amino acids or polyalanine stretch in H6-H7 showed indistinguishable pattern of repression from wild-type SHP. Interestingly, the swapped SHP mutant with DAX-1 extra amino acids interacted with EID-1 (E1A-like inhibitor of differentiation 1), which is characterized as an SHP-interacting corepressor. However, interaction between SHP Delta128-139 and EID-1 was significantly diminished. Moreover, SHP-mediated repression of constitutive androstane receptor transactivation was significantly released by down-regulation of EID-1 expression with EID-1 small interfering RNA. The present study suggests that H6-H7 loop regions of SHP and DAX-1 play a different role in the repression of nuclear receptor transactivation | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1082~1095 | - |
dc.relation.isPartOf | MOLECULAR ENDOCRINOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | COS Cells | - |
dc.subject.MESH | Cercopithecus aethiops | - |
dc.subject.MESH | DAX-1 Orphan Nuclear Receptor | - |
dc.subject.MESH | DNA-Binding Proteins/genetics* | - |
dc.subject.MESH | DNA-Binding Proteins/metabolism | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Models, Molecular* | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Mutation/genetics | - |
dc.subject.MESH | Receptors, Cytoplasmic and Nuclear/genetics* | - |
dc.subject.MESH | Receptors, Cytoplasmic and Nuclear/metabolism | - |
dc.subject.MESH | Receptors, Retinoic Acid/genetics* | - |
dc.subject.MESH | Receptors, Retinoic Acid/metabolism | - |
dc.subject.MESH | Repressor Proteins/genetics* | - |
dc.subject.MESH | Repressor Proteins/metabolism | - |
dc.subject.MESH | Steroidogenic Factor 1 | - |
dc.subject.MESH | Trans-Activators/genetics | - |
dc.subject.MESH | Transcription, Genetic | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.subject.MESH | X Chromosome/genetics* | - |
dc.subject.MESH | X Chromosome/metabolism | - |
dc.title | Differential role of the loop region between helices H6 and H7 within the orphan nuclear receptors small heterodimer partner and DAX-1 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Yun-Yong Park | - |
dc.contributor.googleauthor | Han-Jong Kim | - |
dc.contributor.googleauthor | Hueng-Sik Choi | - |
dc.contributor.googleauthor | Kyoung-Hee Kim | - |
dc.contributor.googleauthor | Minho Shong | - |
dc.contributor.googleauthor | Kang-Yeol Yu | - |
dc.contributor.googleauthor | Ki Cheol Park | - |
dc.contributor.googleauthor | Kwang-Hoon Song | - |
dc.contributor.googleauthor | Mi-Young Kim | - |
dc.contributor.googleauthor | Joon-Young Kim | - |
dc.identifier.doi | 10.1210/me.2003-0339 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00446 | - |
dc.relation.journalcode | J02257 | - |
dc.identifier.eissn | 1944-9917 | - |
dc.identifier.pmid | 14963109 | - |
dc.identifier.url | http://dx.doi.org/10.1210/me.2003-0339 | - |
dc.contributor.alternativeName | Kim, Mi Young | - |
dc.contributor.affiliatedAuthor | Kim, Mi Young | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 18 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1082 | - |
dc.citation.endPage | 1095 | - |
dc.identifier.bibliographicCitation | MOLECULAR ENDOCRINOLOGY , Vol.18(5) : 1082-1095, 2004 | - |
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