Cited 190 times in
Stable stem cell commitment to the adipocyte lineage by inhibition of DNA methylation: Role of the BMP-4 gene
DC Field | Value | Language |
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dc.contributor.author | 김재우 | - |
dc.date.accessioned | 2015-06-10T13:08:04Z | - |
dc.date.available | 2015-06-10T13:08:04Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111071 | - |
dc.description.abstract | Previous studies showed that exposure of C3H10T1/2 stem cells to bone morphogenetic protein-4 (BMP-4) produced cells that convert into adipocytes at high frequency when treated with differentiation inducers. In the present investigation, an independent approach shows that BMP-4 is required for stable commitment of pluripotent stem cells to the adipocyte lineage. Exposure of proliferating 10T1/2 stem cells to 5-azacytidine, a potent DNA methylation inhibitor, gave rise to a subpopulation of cells that can be cloned and that have the capacity to undergo conversion into adipocytes upon treatment with terminal differentiation inducers. Detailed studies performed with a cloned committed subline, the A33 line, verified stable adipocyte lineage determination in the absence of exogenous BMP-4. Remarkably, this cell line expresses and secretes BMP-4 during proliferation in the same time window that exogenous BMP-4 must be added to naïve 10T1/2 cells to induce maximal adipocyte commitment. Furthermore, exposure of A33 cells to noggin, a naturally occurring BMP-4–binding antagonist, during this critical time window blocks subsequent differentiation. The role of BMP-4 in adipocyte lineage commitment is further strengthened by gene expression profiling of proliferating 10T1/2 stem cells and A33 preadipocytes. These findings revealed changes in the molecular circuitry, specifically coordinated changes in the expression of members of the BMP-4 signaling pathway, that distinguish A33 preadipocytes from uncommitted parental 10T1/2 stem cells. Together, these studies provide compelling evidence for the participation of BMP-4 in adipocyte lineage determination. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 13022~13027 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipocytes/cytology* | - |
dc.subject.MESH | Adipocytes/drug effects | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Azacitidine/pharmacology | - |
dc.subject.MESH | Bone Morphogenetic Protein 4 | - |
dc.subject.MESH | Bone Morphogenetic Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Bone Morphogenetic Proteins/genetics* | - |
dc.subject.MESH | Bone Morphogenetic Proteins/metabolism | - |
dc.subject.MESH | Carrier Proteins/metabolism | - |
dc.subject.MESH | Cell Count | - |
dc.subject.MESH | Cell Lineage*/drug effects | - |
dc.subject.MESH | Cell Proliferation/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | DNA Methylation*/drug effects | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mesoderm/cytology | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Phenotype | - |
dc.subject.MESH | Promoter Regions, Genetic/drug effects | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Stem Cells/cytology* | - |
dc.subject.MESH | Stem Cells/drug effects | - |
dc.title | Stable stem cell commitment to the adipocyte lineage by inhibition of DNA methylation: Role of the BMP-4 gene | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Robert R. Bowers | - |
dc.contributor.googleauthor | Jae Woo Kim | - |
dc.contributor.googleauthor | Tamara C. Otto | - |
dc.contributor.googleauthor | M. Daniel Lane | - |
dc.identifier.doi | 10.1073/pnas.0605789103 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00865 | - |
dc.relation.journalcode | J02550 | - |
dc.identifier.eissn | 1091-6490 | - |
dc.identifier.pmid | 16916928 | - |
dc.subject.keyword | 5-azacytidine | - |
dc.subject.keyword | adipogenesis | - |
dc.subject.keyword | determination | - |
dc.subject.keyword | mesenchymal stem cell | - |
dc.subject.keyword | preadipocyte | - |
dc.contributor.alternativeName | Kim, Jae Woo | - |
dc.contributor.affiliatedAuthor | Kim, Jae Woo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 103 | - |
dc.citation.number | 35 | - |
dc.citation.startPage | 13022 | - |
dc.citation.endPage | 13027 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.103(35) : 13022-13027, 2006 | - |
dc.identifier.rimsid | 51959 | - |
dc.type.rims | ART | - |
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