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사람 중이점막 상피세포에서 Uridine-5’ Triphosphate에 의한칼슘 이동과 점액분비에 미치는 카페인의 효과
DC Field | Value | Language |
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dc.contributor.author | 윤주헌 | - |
dc.date.accessioned | 2015-06-10T13:07:34Z | - |
dc.date.available | 2015-06-10T13:07:34Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1225-035X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111055 | - |
dc.description.abstract | Background and Objectives:Puringeric receptors and their agonists like uridine-5-triphosphate (UTP) and adenosine triphosphate (ATP), regulate mucin secretion in middle ear epithelial cells. In the present study, we examined the effects of purinergic agonists on Ca2+ influx ([Ca2+]i) in normal human middle ear epithelial (NHMEE) cells. We also examined the effect of caffeine, an inositol 1, 4, 5-triphosphate (IP3) inhibitor, on UTP induced [Ca2+]i and mucin secretion in NHMEE cells. Materials and Method:NHMEE cells were stimulated with various purinergic agonists, such as UTP, and [Ca2+]i was measured using a miniature double perfusion chamber. UTP-induced mucin secretion was quantitated by immunoblotting assay. Results:The determined order of purinergic agonist potency with respect to [Ca2+]i was ATP=UTP>2-MeSATP>ADP>> adenosine. UTP-induced mucin secretion was inhibited when the intracellular Ca2+ was removed with 2-bis (2-aminophenoxy) ethane-N, N, N', N'-tetraacetic acid-acetoxymethyl ester. Caffeine suppressed UTP-induced [Ca2+]i, and but inhibited UTP-induced and constitutional mucin secretion. Conclusion:Our results suggest that caffeine may have a therapeutic effect in mucoid otitis media by suppressing mucin secretion. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 263~265 | - |
dc.relation.isPartOf | Korean Journal of Otolaryngology (대한이비인후과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 사람 중이점막 상피세포에서 Uridine-5’ Triphosphate에 의한칼슘 이동과 점액분비에 미치는 카페인의 효과 | - |
dc.title.alternative | Effect of Caffeine on UTP-induced Ca2+ Mobilization and Mucin Secretion in Human Middle Ear Epithelial Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | 최재영 | - |
dc.contributor.googleauthor | 김성헌 | - |
dc.contributor.googleauthor | 정상호 | - |
dc.contributor.googleauthor | 손은진 | - |
dc.contributor.googleauthor | 박헌이 | - |
dc.contributor.googleauthor | 신중욱 | - |
dc.contributor.googleauthor | 윤주헌 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02604 | - |
dc.relation.journalcode | J02088 | - |
dc.subject.keyword | Mucin | - |
dc.subject.keyword | Caffeine | - |
dc.subject.keyword | Calcium | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 49 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 263 | - |
dc.citation.endPage | 265 | - |
dc.identifier.bibliographicCitation | Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.49(3) : 263-265, 2006 | - |
dc.identifier.rimsid | 51949 | - |
dc.type.rims | ART | - |
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