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Lysophosphatidylcholine Attenuates Endothelium-dependent Relaxation Responses through Inhibition of ACh-induced Endothelial [Ca2+]i Increase
DC Field | Value | Language |
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dc.contributor.author | 남택상 | - |
dc.contributor.author | 안덕선 | - |
dc.contributor.author | 이영호 | - |
dc.date.accessioned | 2015-06-10T13:07:26Z | - |
dc.date.available | 2015-06-10T13:07:26Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111051 | - |
dc.description.abstract | Lysophosphatidylcholine (LPC), which accumulates in atherosclerotic arteries, has been reported to inhibit endothelium-dependent relaxation (EDR) in many different species. However, the underlying mechanism of LPC-induced inhibition of EDR is still uncertain. In the present study, we measured simultaneously both isometric tension and cytosolic free Ca2+ ([Ca2+]i) in rabbit carotid strips, and examined the effect of LPC on tension and [Ca2+]i. In carotid strips with intact-endothelium, high K+ (70 mM) increased both tension and [Ca2+]i, and cumulative addition of acetylcholine (ACh) from 0.1 to 10μM induced dose dependent increase of [Ca2+]i with concomitant relaxation. In the presence of L-NAME (0.1 mM), ACh increased [Ca2+]i without affecting the amplitude of high K+-induced tension. These ACh-induced change of [Ca2+]i and tension was abolished by removal of endothelium or 10 nM 4-DAMP (muscarinic receptor antagonist) pretreatment. Pretreatment of LPC (10μM) inhibited ACh (10μM)-induced change of tension and [Ca2+]i in endothelium-intact carotid artery. On the other hand, LPC had no effect on ACh-induced change of tension and [Ca2+]i in endothelium denuded artery. In Ca2+-free external solution, ACh transiently increased [Ca2+]i, and pretreatment of LPC significantly inhibited ACh-induced transient [Ca2+]i change. Based on the above results, it may be concluded that LPC inhibits the ACh-induced [Ca2+]i change through inhibition of Ca2+ mobilization in vascular endothelial cells, resulting in decreased production of NO and concomitant inhibition of endothelium-dependent vascular relaxation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Lysophosphatidylcholine Attenuates Endothelium-dependent Relaxation Responses through Inhibition of ACh-induced Endothelial [Ca2+]i Increase | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Seong-Chun Kwon | - |
dc.contributor.googleauthor | Yong-Ho Lee | - |
dc.contributor.googleauthor | Taicksang Nam | - |
dc.contributor.googleauthor | Duck-Sun Ahn | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01271 | - |
dc.contributor.localId | A02223 | - |
dc.contributor.localId | A02968 | - |
dc.relation.journalcode | J02104 | - |
dc.identifier.eissn | 2093-3827 | - |
dc.subject.keyword | Vascular endothelium | - |
dc.subject.keyword | LPC | - |
dc.subject.keyword | EDR | - |
dc.subject.keyword | Endothelial [Ca2+]i | - |
dc.contributor.alternativeName | Nam, Taick Sang | - |
dc.contributor.alternativeName | Ahn, Duk Sun | - |
dc.contributor.alternativeName | Lee, Young Ho | - |
dc.contributor.affiliatedAuthor | Nam, Taick Sang | - |
dc.contributor.affiliatedAuthor | Ahn, Duk Sun | - |
dc.contributor.affiliatedAuthor | Lee, Young Ho | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 25 | - |
dc.citation.endPage | 30 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, Vol.10(1) : 25-30, 2006 | - |
dc.identifier.rimsid | 51945 | - |
dc.type.rims | ART | - |
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