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The interaction of hepatitis B virus X protein and protein phosphatase type 2 C alpha and its effect on IL-6

 Ji Su Kim  ;  Bo young Rho  ;  Tae Ho Lee  ;  Jae Myun Lee  ;  Se Jong Kim  ;  Jeon Han Park 
 Biochemical and Biophysical Research Communications, Vol.351(1) : 253-258, 2006 
Journal Title
 Biochemical and Biophysical Research Communications 
Issue Date
PP2Cα ; HBx ; IL-6 ; Hepatocellular carcinoma
HBx has been suggested as an important determinant mediating the pathological effects of HBV via interacting with various cellular proteins. To identify new HBx-interacting proteins and elucidate a possible mechanism associated with HBx and HBx-interacting proteins in hepatocellular carcinoma, yeast two-hybrid screening was performed. We identified a novel HBx-interacting protein, serine/threonine protein phosphatase PP2Cα, and investigated the effects of PP2Cα on HBx-mediated IL-6 regulation. The interaction between endogenous PP2Cα, and HBx was confirmed by co-immunoprecipitation. Recombinant HBx dose-dependently reduced enzyme activity of recombinant PP2Cα in vitro. While ectopically expressed PP2Cα in Cos-7 and Huh-7 cells reduced the expression of IL-6, overexpressed HBx with recombinant HBx-expressing adenovirus overcame PP2Cα-mediated IL-6 downregulation. In the response of IL-6, HBx phosphorylated STAT3 and recovered PP2Cα-mediated dephosphorylation of STAT3. These results supported that HBx might play a crucial role in HBV-associated hepatocarcinogenesis even in cases where cells express a negative regulator, PP2Cα.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Lee, Jae Myun(이재면) ORCID logo https://orcid.org/0000-0002-5273-3113
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