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EBV Encoded RNA 양성 위암에서 EBV Latent Membrane Protein 1 유전자 변화와 TGF-β1, TGF-βRII, p21,p16, E2F1, Thymidylate Synthase 및 NF-κB 단백 발현에 대한 면역조직화학적 연구
DC Field | Value | Language |
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dc.contributor.author | 김명수 | - |
dc.date.accessioned | 2015-06-10T13:02:30Z | - |
dc.date.available | 2015-06-10T13:02:30Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1738-1843 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/110900 | - |
dc.description.abstract | Background : Although clinicopathologic differences have been described between Epstein- Barr virus (EBV)-positive and negative gastric adenocarcinomas, the pathogenetic basis for these differences remains unclear. In this study, efforts were made to confirm that expression of EBV-latent membrane protein (LMP1) and immunohistochemical characteristics of EBVpositive gastric adenocarcinomas. Methods : We investigated genomic deletion, and RNA & protein expression of the EBV-LMP1, as well as immunohistochemical protein expression of transforming growth factor (TGF)-β1, TGF-βRII, p21, p16, E2F1, thymidylate synthase, and NF-κB in relation to EBV positive gastric adenocarcinoma. Results : A total of 38 Epstein-Barr Virus Encoded RNA-positive and 80 negative gastric carcinomas were examined. A 30 bp DNA deletion in the EBV-LMP1 gene, initiating at codon 342, was detected in 94.4% of EBVpositive cases. By RT-PCR and western blotting, EBV-LMP1 mRNA and protein expressions were absent in all cases, re- gardless of DNA deletion. No significant differences in TGF-β1, TGF-βRII, p21, NF-κB, E2F1, or thymidylate synthase expression were identified. However, the decreased expression of p16 was found in 84.2% of EBV-positive carcinomas, relative to only 57.5% of EBV-negative tumors (p=0.024). Conclusion : EBV-LMP1 DNA deletion, mRNA and protein losses are highly prevalent in EBV-positive gastric adenocarcinoma among Korean patients, along with decreased p16 expression. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 176~184 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PATHOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | EBV Encoded RNA 양성 위암에서 EBV Latent Membrane Protein 1 유전자 변화와 TGF-β1, TGF-βRII, p21,p16, E2F1, Thymidylate Synthase 및 NF-κB 단백 발현에 대한 면역조직화학적 연구 | - |
dc.title.alternative | Genetic Analysis of EBV-LMP1 and Immunohistochemical Expression of TGF-β1, TGF-βRII, p21, p16, E2F1, Thymidylate Synthase, and NF-κB in EBV Encoded RNA-positive Gastric Adenocarcinoma | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | Cho Mee-Yon | - |
dc.contributor.googleauthor | Eom Min-seob | - |
dc.contributor.googleauthor | Park Kwang-Hwa | - |
dc.contributor.googleauthor | Kim Mee-Dong | - |
dc.contributor.googleauthor | Sung Seung-Hoon | - |
dc.contributor.googleauthor | Kim Myoung-Soo | - |
dc.contributor.googleauthor | Kim Dae-Sung | - |
dc.contributor.googleauthor | Choi Sun-Ju | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00424 | - |
dc.relation.journalcode | J02092 | - |
dc.identifier.eissn | 2092-8920 | - |
dc.subject.keyword | Stomach neoplasms | - |
dc.subject.keyword | Epstein-Barr virus | - |
dc.subject.keyword | EBV-Associated Membrane Antigen | - |
dc.subject.keyword | Protein p16 | - |
dc.contributor.alternativeName | Kim, Myoung Soo | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Soo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 40 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 176 | - |
dc.citation.endPage | 184 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PATHOLOGY , Vol.40(3) : 176-184, 2006 | - |
dc.identifier.rimsid | 55604 | - |
dc.type.rims | ART | - |
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