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Bio-rad elite microplate 장비를 이용한 HBs 항원, HCV, HIV 항원-항체 검사시약 평가
DC Field | Value | Language |
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dc.contributor.author | 김현숙 | - |
dc.date.accessioned | 2015-06-10T12:54:28Z | - |
dc.date.available | 2015-06-10T12:54:28Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1598-6535 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/110664 | - |
dc.description.abstract | Background: In this study, we evaluated the performance of Elite microplate analyzer (Bio-Rad Laboratories, France) and the related assays (ULTRA line) for the detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV). Methods: Seroconversion panels, HBsAg positive/HBsAg negative (n=99/n=1,422), anti-HCV positive/negative (n=97/n=1,670), and anti-HIV positive/negative (n=112/n=1,704) samples were used to evaluate the performance of Monolisa HBsAg ULTRA, Monolisa HCV Ag-Ab ULTRA, and Genscreen ULTRA HIV Ag-Ab, respectively. The agreement of Elite microplate analyzer with CODA analyzer (Bio-Rad Laboratories, CA, USA) was also evaluated. Results: The detection limit of Monolisa HBsAg ULTRA was 0.034 IU/mL. For Monolisa HBsAg ULTRA, Monolisa HCV Ag-Ab ULTRA, and Genscreen ULTRA HIV Ag-Ab, diagnostic sensitivities were all 100%, diagnostic specificities were 100%, 99.8% and 99.9%, and total CVs (coefficients of variation) were 13.8-17.5%, 3.4-5.2%, and 7.5-9.5%, respectively. The agreement of Elite microplate analyzer with CODA analyzer was 99.5%. Conclusion: The performance of Elite microplate analyzer and the related assays on analytical sensitivity, precision, early detection, diagnostic sensitivity and specificity was all adequate for a mass screening. However, further large multi-center studies should be performed to validate our results. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 436~441 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF LABORATORY MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Bio-rad elite microplate 장비를 이용한 HBs 항원, HCV, HIV 항원-항체 검사시약 평가 | - |
dc.title.alternative | Evaluation of HBs Ag, HCV and HIV Ag-Ab Assays using Bio-Rad Elite Microplate Analyzer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | 황상현 | - |
dc.contributor.googleauthor | 오흥범 | - |
dc.contributor.googleauthor | 김현숙 | - |
dc.contributor.googleauthor | 이은엽 | - |
dc.identifier.doi | 10.3343/kjlm.2006.26.6.436 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01117 | - |
dc.relation.journalcode | J02044 | - |
dc.identifier.pmid | 18156764 | - |
dc.subject.keyword | Hepatitis B virus | - |
dc.subject.keyword | Hepatitis C virus | - |
dc.subject.keyword | Human immunodeficiency virus | - |
dc.subject.keyword | Evaluation | - |
dc.subject.keyword | Sensitivity | - |
dc.subject.keyword | Specificity | - |
dc.subject.keyword | Elite Microplate Analyzer | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 26 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 436 | - |
dc.citation.endPage | 441 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.26(6) : 436-441, 2006 | - |
dc.identifier.rimsid | 57529 | - |
dc.type.rims | ART | - |
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