Cited 11 times in
Effects of prostagladin E2 on gel-forming mucin secretion in normal human nasal epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 김창훈 | - |
dc.contributor.author | 윤주헌 | - |
dc.date.accessioned | 2015-06-10T12:45:23Z | - |
dc.date.available | 2015-06-10T12:45:23Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0001-6489 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/110388 | - |
dc.description.abstract | Conclusion. The findings of this study indicate that prostaglandin E2 (PGE2) induces MUC5AC gene expression and mucin secretion via the EP4 receptor in cultured normal human nasal epithelial cells. Objectives. Recently, PGE2 was found to induce MUC5AC production via an agonist of EP2/EP4, but not EP1/EP3, in normal human airway epithelium. However, the receptor that mediates MUC5AC has not been determined. This study aimed to investigate the MUC5AC mucin gene and mucin secretion by PGE2 and its receptors in cultured normal human nasal epithelial cells. Methods. After treatment with PGE2 and/or PGE2 antagonist, gel-forming mucin mRNA expression was determined by reverse transcription-polymerase chain reaction. Total mucin and MUC5AC mucin levels were measured using an immuno-dot blotting assay. Results. PGE2 increased only MUC5AC gene expression and MUC5AC mucin, but not expression of other gel-forming mucin genes. An EP2 receptor antagonist (AH 6809) did not suppress the PGE2-induced MUC5AC gene expression or MUC5AC mucin. However, an EP4 receptor antagonist (AH23848) significantly suppressed the level of PGE2-induced MUC5AC gene expression and MUC5AC mucin. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 174~179 | - |
dc.relation.isPartOf | ACTA OTO-LARYNGOLOGICA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Dinoprostone/pharmacology* | - |
dc.subject.MESH | Gene Expression/drug effects* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Mucin 5AC | - |
dc.subject.MESH | Mucins/biosynthesis | - |
dc.subject.MESH | Mucins/drug effects | - |
dc.subject.MESH | Mucins/genetics | - |
dc.subject.MESH | Mucins/metabolism* | - |
dc.subject.MESH | Nasal Mucosa/cytology | - |
dc.subject.MESH | Nasal Mucosa/metabolism* | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Receptors, Prostaglandin E/biosynthesis* | - |
dc.subject.MESH | Receptors, Prostaglandin E/drug effects | - |
dc.subject.MESH | Receptors, Prostaglandin E/genetics | - |
dc.subject.MESH | Receptors, Prostaglandin E, EP2 Subtype | - |
dc.subject.MESH | Receptors, Prostaglandin E, EP4 Subtype | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.title | Effects of prostagladin E2 on gel-forming mucin secretion in normal human nasal epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | Jin Kook Kim | - |
dc.contributor.googleauthor | Chang Hoon Kim | - |
dc.contributor.googleauthor | Kyubo Kim | - |
dc.contributor.googleauthor | Hyun Jong Jang | - |
dc.contributor.googleauthor | Hyun Jik Kim | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1080/00016480500280033 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01050 | - |
dc.contributor.localId | A02604 | - |
dc.relation.journalcode | J00028 | - |
dc.identifier.eissn | 1651-2251 | - |
dc.identifier.pmid | 16428196 | - |
dc.identifier.url | http://informahealthcare.com/doi/abs/10.1080/00016480500280033 | - |
dc.subject.keyword | Prostaglandin E2 | - |
dc.subject.keyword | mucin | - |
dc.subject.keyword | secretion | - |
dc.subject.keyword | prostaglandin receptor | - |
dc.contributor.alternativeName | Kim, Chang Hoon | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Kim, Chang Hoon | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 126 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 174 | - |
dc.citation.endPage | 179 | - |
dc.identifier.bibliographicCitation | ACTA OTO-LARYNGOLOGICA, Vol.126(2) : 174-179, 2006 | - |
dc.identifier.rimsid | 38756 | - |
dc.type.rims | ART | - |
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