Cited 9 times in
Liver-directed gene therapy of diabetic rats using an HVJ-E vector containing EBV plasmids expressing insulin and GLUT 2 transporter
DC Field | Value | Language |
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dc.contributor.author | 이현철 | - |
dc.date.accessioned | 2015-06-10T12:38:07Z | - |
dc.date.available | 2015-06-10T12:38:07Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/110168 | - |
dc.description.abstract | Insulin gene therapy in clinical medicine is currently hampered by the inability to regulate insulin secretion in a physiological manner, the inefficiency with which the gene is delivered, and the short duration of gene expression. To address these issues, we injected the liver of streptozotocin-induced diabetic rats with hemagglutinating virus of Japan-envelope (HVJ-E) vectors containing Epstein–Barr virus (EBV) plasmids encoding the genes for insulin and the GLUT 2 transporter. Efficient delivery of the genes was achieved with the HVJ-E vector, and the use of the EBV replicon vector led to prolonged hepatic gene expression. Blood glucose levels were normalized for at least 3 weeks as a result of the gene therapy. Cotransfection of GLUT 2 with insulin permitted the diabetic rats to regulate their blood glucose levels upon exogenous glucose loading in a physiologically appropriate manner and improved postprandial glucose levels. Moreover, cotransfection with insulin and GLUT 2 genes led to in vitro glucose-stimulated insulin secretion that involved the closure of KATP channels. The present study represents a new way to efficiently deliver insulin gene in vivo that is regulated by ambient glucose level with prolonged gene expression. This may provide a basis to overcome limitations of insulin gene therapy in humans. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 216~224 | - |
dc.relation.isPartOf | GENE THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Diabetes Mellitus, Experimental/metabolism | - |
dc.subject.MESH | Diabetes Mellitus, Experimental/therapy* | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Genetic Therapy/methods* | - |
dc.subject.MESH | Genetic Vectors/administration & dosage* | - |
dc.subject.MESH | Genetic Vectors/genetics | - |
dc.subject.MESH | Glucose Transporter Type 2/genetics* | - |
dc.subject.MESH | Glucose Transporter Type 2/metabolism | - |
dc.subject.MESH | Herpesvirus 4, Human/genetics | - |
dc.subject.MESH | Insulin/genetics* | - |
dc.subject.MESH | Insulin/metabolism | - |
dc.subject.MESH | Liver/metabolism* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Potassium Channels/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Sendai virus/genetics | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Transduction, Genetic | - |
dc.subject.MESH | Viral Envelope Proteins/genetics | - |
dc.title | Liver-directed gene therapy of diabetic rats using an HVJ-E vector containing EBV plasmids expressing insulin and GLUT 2 transporter | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Y D Kim | - |
dc.contributor.googleauthor | K-G Park | - |
dc.contributor.googleauthor | R Morishita | - |
dc.contributor.googleauthor | Y Kaneda | - |
dc.contributor.googleauthor | S-Y Kim | - |
dc.contributor.googleauthor | D-K Song | - |
dc.contributor.googleauthor | H-S Kim | - |
dc.contributor.googleauthor | C-W Nam | - |
dc.contributor.googleauthor | H C Lee | - |
dc.contributor.googleauthor | K-U Lee | - |
dc.contributor.googleauthor | J-Y Park | - |
dc.contributor.googleauthor | B-W Kim | - |
dc.contributor.googleauthor | J-G Kim | - |
dc.contributor.googleauthor | I-K Lee | - |
dc.identifier.doi | 10.1038/sj.gt.3302644 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03301 | - |
dc.relation.journalcode | J00924 | - |
dc.identifier.eissn | 1476-5462 | - |
dc.identifier.pmid | 16177820 | - |
dc.identifier.url | http://www.nature.com/gt/journal/v13/n3/full/3302644a.html | - |
dc.contributor.alternativeName | Lee, Hyun Chul | - |
dc.contributor.affiliatedAuthor | Lee, Hyun Chul | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 13 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 216 | - |
dc.citation.endPage | 224 | - |
dc.identifier.bibliographicCitation | GENE THERAPY, Vol.13(2) : 216-224, 2006 | - |
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