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Elevated Contractile Responses to Acetylcholine in Organ Cultured Rabbit Carotid Artery
DC Field | Value | Language |
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dc.contributor.author | 남택상 | - |
dc.contributor.author | 안덕선 | - |
dc.contributor.author | 원종은 | - |
dc.contributor.author | 이영호 | - |
dc.contributor.author | 정승수 | - |
dc.date.accessioned | 2015-06-10T12:32:16Z | - |
dc.date.available | 2015-06-10T12:32:16Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109985 | - |
dc.description.abstract | The aim of the present study was to examine the functional changes that occur when a rabbit carotid artery is cultured in serum-free medium. In endothelium (EC)-intact arteries cultured under serum-free conditions, acetylcholine (ACh)-induced relaxation responses were partially, yet significantly, reduced when compared with freshly isolated arteries. After pretreatment with NG-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, application of ACh resulted in a significant contraction in organ cultured arteries. The amplitude of the ACh-induced contractions increased with the duration of culture. In EC-denuded arteries cultured under serum-free conditions, ACh induced responses similar to those in EC-intact arteries pretreated with L-NAME. Furthermore, ACh caused a significant increase in intracellular Ca2+ concentration ([Ca2+]i) in EC-denuded arteries cultured under serum-free condition for 7 days. There was little change in either [Ca2+]i or tension in freshly isolated carotid rings. There was no difference in sodium nitroprusside-induced relaxation responses between fresh and cultured arteries. These results suggest that prolonged culture of carotid arteries under serum-free conditions changes the functional properties of vascular reactivity in rabbit carotid arteries. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 249~254 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acetylcholine/pharmacology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Carotid Arteries/drug effects* | - |
dc.subject.MESH | Carotid Arteries/metabolism | - |
dc.subject.MESH | Carotid Arteries/pathology* | - |
dc.subject.MESH | Culture Media, Serum-Free/metabolism | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Models, Statistical | - |
dc.subject.MESH | Muscle Contraction* | - |
dc.subject.MESH | NG-Nitroarginine Methyl Ester/metabolism | - |
dc.subject.MESH | NG-Nitroarginine Methyl Ester/pharmacology | - |
dc.subject.MESH | Nitroprusside/pharmacology | - |
dc.subject.MESH | Organ Culture Techniques/methods* | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Time Factors | - |
dc.title | Elevated Contractile Responses to Acetylcholine in Organ Cultured Rabbit Carotid Artery | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Youngho Lee | - |
dc.contributor.googleauthor | Seungsoo Jung | - |
dc.contributor.googleauthor | Jong-eun Won | - |
dc.contributor.googleauthor | Taiksang Nam | - |
dc.contributor.googleauthor | Ducksun Ahn | - |
dc.identifier.doi | 10.3349/ymj.2006.47.2.249 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01271 | - |
dc.contributor.localId | A02223 | - |
dc.contributor.localId | A02444 | - |
dc.contributor.localId | A02968 | - |
dc.contributor.localId | A03643 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 16642556 | - |
dc.subject.keyword | Organ culture | - |
dc.subject.keyword | carotid artery | - |
dc.subject.keyword | acetylcholine | - |
dc.subject.keyword | NG-nitro-L-arginine methyl ester | - |
dc.subject.keyword | Ca2+ regulation | - |
dc.contributor.alternativeName | Nam, Taick Sang | - |
dc.contributor.alternativeName | Ahn, Duk Sun | - |
dc.contributor.alternativeName | Won, Jong Eun | - |
dc.contributor.alternativeName | Lee, Young Ho | - |
dc.contributor.alternativeName | Chung, Seung Soo | - |
dc.contributor.affiliatedAuthor | Nam, Taick Sang | - |
dc.contributor.affiliatedAuthor | Ahn, Duk Sun | - |
dc.contributor.affiliatedAuthor | Won, Jong Eun | - |
dc.contributor.affiliatedAuthor | Lee, Young Ho | - |
dc.contributor.affiliatedAuthor | Chung, Seung Soo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 47 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 249 | - |
dc.citation.endPage | 254 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.47(2) : 249-254, 2006 | - |
dc.identifier.rimsid | 55071 | - |
dc.type.rims | ART | - |
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