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Elevated Contractile Responses to Acetylcholine in Organ Cultured Rabbit Carotid Artery

 Youngho Lee  ;  Seungsoo Jung  ;  Jong-eun Won  ;  Taiksang Nam  ;  Ducksun Ahn 
 Yonsei Medical Journal, Vol.47(2) : 249-254, 2006 
Journal Title
 Yonsei Medical Journal 
Issue Date
The aim of the present study was to examine the functional changes that occur when a rabbit carotid artery is cultured in serum-free medium. In endothelium (EC)-intact arteries cultured under serum-free conditions, acetylcholine (ACh)-induced relaxation responses were partially, yet significantly, reduced when compared with freshly isolated arteries. After pretreatment with NG-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, application of ACh resulted in a significant contraction in organ cultured arteries. The amplitude of the ACh-induced contractions increased with the duration of culture. In EC-denuded arteries cultured under serum-free conditions, ACh induced responses similar to those in EC-intact arteries pretreated with L-NAME. Furthermore, ACh caused a significant increase in intracellular Ca2+ concentration ([Ca2+]i) in EC-denuded arteries cultured under serum-free condition for 7 days. There was little change in either [Ca2+]i or tension in freshly isolated carotid rings. There was no difference in sodium nitroprusside-induced relaxation responses between fresh and cultured arteries. These results suggest that prolonged culture of carotid arteries under serum-free conditions changes the functional properties of vascular reactivity in rabbit carotid arteries.
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1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
남택상(Nam, Taick Sang)
안덕선(Ahn, Duk Sun) ORCID logo https://orcid.org/0000-0001-9351-6951
원종은(Won, Jong Eun)
이영호(Lee, Young Ho) ORCID logo https://orcid.org/0000-0002-5749-1045
정승수(Chung, Seung Soo) ORCID logo https://orcid.org/0000-0002-3119-9628
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