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Gene Transfer of Pigment Epithelium-Derived Factor Suppresses Tumor Growth and Angiogenesis in a Hepatoblastoma Xenograft Model

DC FieldValueLanguage
dc.contributor.author이은직-
dc.date.accessioned2015-06-10T12:32:12Z-
dc.date.available2015-06-10T12:32:12Z-
dc.date.issued2006-
dc.identifier.issn0031-3998-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/109983-
dc.description.abstractNormal hepatocytes express pigment epithelium-derived factor (PEDF), an endogenous antiangiogenic factor. We hypothesized that decreased PEDF expression may be one mechanism driving hepatoblastoma growth, and in vivo gene transfer of PEDF could suppress neovascularization and limit tumor growth. PEDF functional activity was determined in vitro using endothelial cell migration assays and in vivo using a subcutaneous tumor model. HUH-6 human hepatoblastoma tumors were treated with hybrid adenoviral/adeno-associated viral expression vectors for PEDF (Hyb-PEDF, n = 4) or β-galactosidase (Hyb-βgal, n = 4) daily for 4 d. Mitotic figures, microvascular density (MVD), PEDF, and VEGF expression were assessed. Hyb-PEDF treatment inhibited in vivo tumor growth (p < 0.008) and decreased MVD (p < 0.001), the number of mitotic figures (p < 0.001), and VEGF expression when compared with Hyb-βgal-treated tumors. HUH-6 expression of PEDF was dramatically reduced when cultured under hypoxic conditions and also when grown in vivo, and the addition of neutralizing anti-PEDF antibody increased the already high baseline angiogenic activity of the HUH-6 cell secretions in vitro (p < 0.04). PEDF is an important endogenous regulator of the liver vasculature. Augmenting intra-tumoral PEDF levels inhibits tumor growth by reducing angiogenesis and VEGF expression. Potent inhibitors of angiogenesis, such as PEDF, may be an effective alternative treatment for children with hepatoblastoma.-
dc.description.statementOfResponsibilityopen-
dc.format.extent282~287-
dc.relation.isPartOfPEDIATRIC RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHEye Proteins/genetics*-
dc.subject.MESHEye Proteins/metabolism-
dc.subject.MESHFemale-
dc.subject.MESHGenetic Therapy*-
dc.subject.MESHHepatoblastoma/therapy*-
dc.subject.MESHHumans-
dc.subject.MESHLiver Neoplasms/therapy*-
dc.subject.MESHMice-
dc.subject.MESHNeovascularization, Pathologic/therapy*-
dc.subject.MESHNerve Growth Factors/genetics*-
dc.subject.MESHNerve Growth Factors/metabolism-
dc.subject.MESHSerpins/genetics*-
dc.subject.MESHSerpins/metabolism-
dc.subject.MESHTransplantation, Heterologous-
dc.titleGene Transfer of Pigment Epithelium-Derived Factor Suppresses Tumor Growth and Angiogenesis in a Hepatoblastoma Xenograft Model-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorMarybeth Browne-
dc.contributor.googleauthorVeronica Stellmach-
dc.contributor.googleauthorMona Cornwell-
dc.contributor.googleauthorChuhan Chung-
dc.contributor.googleauthorJennifer A Doll-
dc.contributor.googleauthorEun-Jig Lee-
dc.contributor.googleauthorJ Larry Jameson-
dc.contributor.googleauthorMarleta Reynolds-
dc.contributor.googleauthorRiccardo A Superina-
dc.contributor.googleauthorLisa P Abramson-
dc.contributor.googleauthorSusan E Crawfor-
dc.identifier.doi10.1203/01.pdr.0000232789.86632.91-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03050-
dc.relation.journalcodeJ02493-
dc.identifier.eissn1530-0447-
dc.identifier.pmid16857775-
dc.identifier.urlhttp://www.nature.com/pr/journal/v60/n3/full/pr2006238a.html-
dc.contributor.alternativeNameLee, Eun Jig-
dc.contributor.affiliatedAuthorLee, Eun Jig-
dc.rights.accessRightsnot free-
dc.citation.volume60-
dc.citation.number3-
dc.citation.startPage282-
dc.citation.endPage287-
dc.identifier.bibliographicCitationPEDIATRIC RESEARCH, Vol.60(3) : 282-287, 2006-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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