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저산소증 상태에서 정상임신과 임신중독증 환자의 태반 조직 내 endothelin-1 (ET-1) 발현의 비교
DC Field | Value | Language |
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dc.contributor.author | 김영한 | - |
dc.contributor.author | 박용원 | - |
dc.date.accessioned | 2015-06-10T12:31:38Z | - |
dc.date.available | 2015-06-10T12:31:38Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 2287-8572 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109965 | - |
dc.description.abstract | Objective: To evaluate endothelin-1 (ET-1) expression in the villous explants from normal and preeclamptic (PE) placentae under hypoxic condition. Methods: Villous explants from normal (n=5) and PE (n=4) placentae were obtained. To obtain hypoxic culture condition, villous explants were cultured in hypoxic chamber or treated with deferoxamine (DFO). ET-1 mRNA expressions in villous explants were evaluated by RT-PCR following 0, 24, and 48 h of culture in hypoxic chamber, and 0, 2, 4, 6 h following DFO treatment. ET-1 protein levels in media were measured by enzyme immunoassay. Results: After 24 and 48 hours of incubation of villous explants from normal and PE placentae in hypoxic chamber, ET-1 mRNA and protein levels were increased in both groups, however, ET-1 production seemed to be more exaggerated in the villous explants from PE placentae. During 6 h of DFO exposure, ET-1 mRNA level was increased in the villous explants from PE placenta comparing to those from normal placentae (p<0.05). Interestingly, the increase of ET-1 mRNA expression in the villous explants from PE placentae was more exaggerated than those from normal placentae. Concordantly, increments of protein level between 0 to 2 h and 2 to 4 h were significantly higher in villous explants from PE placentae (p<0.05). Conclusion: ET-1 mRNA and protein were increased in villous explants from PE placentae compared to those from normal placentae under hypoxic condition. Furthermore, villous explants from PE placentae showed upregulated ET-1 expression upon hypoxic stimulation. This enhanced sensitivity to hypoxia may contribute to ET-1 overexpression in PE placenta in vivo and it needs further investigation for clarification. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1844~1854 | - |
dc.relation.isPartOf | Korean Journal of Obstetrics and Gynecology (대한산부인과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 저산소증 상태에서 정상임신과 임신중독증 환자의 태반 조직 내 endothelin-1 (ET-1) 발현의 비교 | - |
dc.title.alternative | Comparison of placental endothelin-1 (ET-1) expression under hypoxic condition between normal versus preeclamptic pregnancy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Obstetrics & Gynecology (산부인과학) | - |
dc.contributor.googleauthor | 박용원 | - |
dc.contributor.googleauthor | 권자영 | - |
dc.contributor.googleauthor | 김영한 | - |
dc.contributor.googleauthor | 김유리 | - |
dc.contributor.googleauthor | 임재학 | - |
dc.contributor.googleauthor | 강명화 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00730 | - |
dc.contributor.localId | A01581 | - |
dc.relation.journalcode | J02076 | - |
dc.subject.keyword | Preeclampsia | - |
dc.subject.keyword | Endothelin-1 | - |
dc.subject.keyword | Hypoxia | - |
dc.contributor.alternativeName | Kim, Young Han | - |
dc.contributor.alternativeName | Park, Yong Won | - |
dc.contributor.affiliatedAuthor | Kim, Young Han | - |
dc.contributor.affiliatedAuthor | Park, Yong Won | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 49 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1844 | - |
dc.citation.endPage | 1854 | - |
dc.identifier.bibliographicCitation | Korean Journal of Obstetrics and Gynecology (대한산부인과학회지), Vol.49(9) : 1844-1854, 2006 | - |
dc.identifier.rimsid | 55053 | - |
dc.type.rims | ART | - |
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