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Novel monoclonal antibody that recognizes new neoantigenic determinant of D-dimer

DC FieldValueLanguage
dc.contributor.author송경순-
dc.contributor.author장양수-
dc.date.accessioned2015-06-10T12:29:16Z-
dc.date.available2015-06-10T12:29:16Z-
dc.date.issued2006-
dc.identifier.issn0049-3848-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/109892-
dc.description.abstractOur novel monoclonal antibody (mAb) B4 reacted with only D-dimer but not intact fibrinogen, or fibrinogen degradation products (FgDP) such as D-monomer, E fragment on ELISA. B4 didn't react with denatured D-dimer, while it reacted well with denatured D-monomer rather than the native form, indicating that B4 recognizes some neoconformational epitope in D-dimer. In our epitope study, B4 recognized the N-terminal (Bβ134-142) of D-dimer, which corresponds to the most flexible segment of coiled coil backbone. It was confirmed by inhibition assay of B4 binding to D-dimer using the synthesized peptides with this sequence. As the other evidence, B4 didn't bind to some D-dimer species produced from a particular fibrinogen variant. This fibrinogen variant is mutated BβLys133 residue to Gln133 thus it doesn't produce the particular N-terminal epitope of D134∼ by plasmin. Finally, our mAb was useful for clinical application. ELISA using our mAbs was well correlated with other commercial D-dimer ELISAs and in some clinical samples it was preferable to them. These results suggest that the epitope for B4 is another neoantigenic determinant in native D-dimer as distinct from native D-monomer.-
dc.description.statementOfResponsibilityopen-
dc.format.extent353~360-
dc.relation.isPartOfTHROMBOSIS RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAntibodies, Monoclonal/chemistry*-
dc.subject.MESHAntibodies, Monoclonal/immunology*-
dc.subject.MESHBinding Sites-
dc.subject.MESHEpitope Mapping*-
dc.subject.MESHEpitopes/immunology*-
dc.subject.MESHFibrin Fibrinogen Degradation Products/chemistry*-
dc.subject.MESHFibrin Fibrinogen Degradation Products/immunology*-
dc.subject.MESHHumans-
dc.subject.MESHProtein Binding-
dc.titleNovel monoclonal antibody that recognizes new neoantigenic determinant of D-dimer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorHyun-Ju Doh-
dc.contributor.googleauthorKyung-Soon Song-
dc.contributor.googleauthorMyung-Seo Kang-
dc.contributor.googleauthorDoo-Sik Kim-
dc.contributor.googleauthorKyung-Ah Kim-
dc.contributor.googleauthorJemo Kang-
dc.contributor.googleauthorYangsoo Jang-
dc.contributor.googleauthorKwang-Hoe Chung-
dc.identifier.doi10.1016/j.thromres.2005.07.024-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02011-
dc.contributor.localIdA03448-
dc.relation.journalcodeJ02727-
dc.identifier.eissn1879-2472-
dc.identifier.pmid16139872-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0049384805003191-
dc.subject.keywordNeo-epitope-
dc.subject.keywordD-dimer-
dc.subject.keywordMonoclonal antibody-
dc.subject.keywordD-monomer-
dc.contributor.alternativeNameSong, Kyung Soon-
dc.contributor.alternativeNameJang, Yang Soo-
dc.contributor.affiliatedAuthorSong, Kyung Soon-
dc.contributor.affiliatedAuthorJang, Yang Soo-
dc.rights.accessRightsnot free-
dc.citation.volume118-
dc.citation.number3-
dc.citation.startPage353-
dc.citation.endPage360-
dc.identifier.bibliographicCitationTHROMBOSIS RESEARCH, Vol.118(3) : 353-360, 2006-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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