Cited 5 times in
Novel monoclonal antibody that recognizes new neoantigenic determinant of D-dimer
DC Field | Value | Language |
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dc.contributor.author | 송경순 | - |
dc.contributor.author | 장양수 | - |
dc.date.accessioned | 2015-06-10T12:29:16Z | - |
dc.date.available | 2015-06-10T12:29:16Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0049-3848 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109892 | - |
dc.description.abstract | Our novel monoclonal antibody (mAb) B4 reacted with only D-dimer but not intact fibrinogen, or fibrinogen degradation products (FgDP) such as D-monomer, E fragment on ELISA. B4 didn't react with denatured D-dimer, while it reacted well with denatured D-monomer rather than the native form, indicating that B4 recognizes some neoconformational epitope in D-dimer. In our epitope study, B4 recognized the N-terminal (Bβ134-142) of D-dimer, which corresponds to the most flexible segment of coiled coil backbone. It was confirmed by inhibition assay of B4 binding to D-dimer using the synthesized peptides with this sequence. As the other evidence, B4 didn't bind to some D-dimer species produced from a particular fibrinogen variant. This fibrinogen variant is mutated BβLys133 residue to Gln133 thus it doesn't produce the particular N-terminal epitope of D134∼ by plasmin. Finally, our mAb was useful for clinical application. ELISA using our mAbs was well correlated with other commercial D-dimer ELISAs and in some clinical samples it was preferable to them. These results suggest that the epitope for B4 is another neoantigenic determinant in native D-dimer as distinct from native D-monomer. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 353~360 | - |
dc.relation.isPartOf | THROMBOSIS RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Antibodies, Monoclonal/chemistry* | - |
dc.subject.MESH | Antibodies, Monoclonal/immunology* | - |
dc.subject.MESH | Binding Sites | - |
dc.subject.MESH | Epitope Mapping* | - |
dc.subject.MESH | Epitopes/immunology* | - |
dc.subject.MESH | Fibrin Fibrinogen Degradation Products/chemistry* | - |
dc.subject.MESH | Fibrin Fibrinogen Degradation Products/immunology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Protein Binding | - |
dc.title | Novel monoclonal antibody that recognizes new neoantigenic determinant of D-dimer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Hyun-Ju Doh | - |
dc.contributor.googleauthor | Kyung-Soon Song | - |
dc.contributor.googleauthor | Myung-Seo Kang | - |
dc.contributor.googleauthor | Doo-Sik Kim | - |
dc.contributor.googleauthor | Kyung-Ah Kim | - |
dc.contributor.googleauthor | Jemo Kang | - |
dc.contributor.googleauthor | Yangsoo Jang | - |
dc.contributor.googleauthor | Kwang-Hoe Chung | - |
dc.identifier.doi | 10.1016/j.thromres.2005.07.024 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02011 | - |
dc.contributor.localId | A03448 | - |
dc.relation.journalcode | J02727 | - |
dc.identifier.eissn | 1879-2472 | - |
dc.identifier.pmid | 16139872 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0049384805003191 | - |
dc.subject.keyword | Neo-epitope | - |
dc.subject.keyword | D-dimer | - |
dc.subject.keyword | Monoclonal antibody | - |
dc.subject.keyword | D-monomer | - |
dc.contributor.alternativeName | Song, Kyung Soon | - |
dc.contributor.alternativeName | Jang, Yang Soo | - |
dc.contributor.affiliatedAuthor | Song, Kyung Soon | - |
dc.contributor.affiliatedAuthor | Jang, Yang Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 118 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 353 | - |
dc.citation.endPage | 360 | - |
dc.identifier.bibliographicCitation | THROMBOSIS RESEARCH, Vol.118(3) : 353-360, 2006 | - |
dc.identifier.rimsid | 50666 | - |
dc.type.rims | ART | - |
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