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Beneficial Effects of Freezing Rate Determined by Indirect Thermophysical Calculation on Cell Viability in Cryopreserved Tissues

DC Field Value Language
dc.contributor.author박영환-
dc.contributor.author박한기-
dc.date.accessioned2015-06-10T12:28:06Z-
dc.date.available2015-06-10T12:28:06Z-
dc.date.issued2006-
dc.identifier.issn1073-1199-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/109856-
dc.description.abstractMany types of mammalian cells, such as sperm, blood, embryos, etc., have been successfully cryopreserved for the last few decades, while no optimal method for the cryopreservation of mammalian tissues or organs has been established, showing a poor survival after thawing with a low recovery of function. In this study, the freezing rate was determined by indirect thermodynamic calculation, and its potential effect on the cryoprotection of human saphenous veins and tissue-engineered bones was investigated. The vein segments were frozen according to the calculated freezing rate, using rate-controlled freezing devices, with a freezing solution composed of 10% dimethylsulphoxide and 20% fetal bovine serum in RPMI 1640 media. The efficacy of indirect calculation was assessed by the cell viability measured using fluorescence double-staining methods. The results indicated that the freezing rate determined by indirect calculation significantly (P < 0.05) maintained the post-thaw cellular viability of the blood vessel, particularly in terms of the endothelial cells. However, it exerted relatively less protective effect on the osteoblastic cell-cultured scaffolds. These results suggest that freezing-induced injuries may occur in tissues, and the freezing rate determined by indirect thermophysical calculation can be used for the optimization of tissue cryopreservation by minimizing the injuries.-
dc.description.statementOfResponsibilityopen-
dc.format.extent205~221-
dc.relation.isPartOfARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBone and Bones*-
dc.subject.MESHCell Survival-
dc.subject.MESHCryopreservation/methods*-
dc.subject.MESHCryopreservation/standards-
dc.subject.MESHFreezing-
dc.subject.MESHHumans-
dc.subject.MESHSaphenous Vein*-
dc.subject.MESHThermodynamics-
dc.titleBeneficial Effects of Freezing Rate Determined by Indirect Thermophysical Calculation on Cell Viability in Cryopreserved Tissues-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Thoracic & Cardiovascular Surgery (흉부외과학)-
dc.contributor.googleauthorDong-Wook Han-
dc.contributor.googleauthorHan-Ki Park-
dc.contributor.googleauthorYoung Hwan Park-
dc.contributor.googleauthorTaek-Soo Kim-
dc.contributor.googleauthorWoong-Sub Yoon-
dc.contributor.googleauthorJeong Koo Kim-
dc.contributor.googleauthorJong-Chul Park-
dc.identifier.doi10.1080/10731190600581742-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01574-
dc.contributor.localIdA01729-
dc.relation.journalcodeJ00245-
dc.identifier.eissn1532-4184-
dc.identifier.pmid16537175-
dc.identifier.urlhttp://informahealthcare.com/doi/abs/10.1080/10731190600581742-
dc.subject.keywordFreezing rate-
dc.subject.keywordIndirect thermodynamic calculation-
dc.subject.keywordCryopreservation-
dc.subject.keywordCell viability-
dc.subject.keywordHuman saphenous veins-
dc.contributor.alternativeNamePark, Young Hwan-
dc.contributor.alternativeNamePark, Han Ki-
dc.contributor.affiliatedAuthorPark, Young Hwan-
dc.contributor.affiliatedAuthorPark, Han Ki-
dc.rights.accessRightsnot free-
dc.citation.volume34-
dc.citation.number2-
dc.citation.startPage205-
dc.citation.endPage221-
dc.identifier.bibliographicCitationARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY , Vol.34(2) : 205-221, 2006-
dc.identifier.rimsid50640-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers

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