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KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line

Authors
 Bo Keun Jee  ;  Koung Min Park  ;  Sibin Surendran  ;  Woon Kyu Lee  ;  Chang Whan Han  ;  Yong Sik Kim  ;  Young Lim 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.342(2) : 655-661, 2006 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2006
MeSH
Carcinoma/enzymology ; Carcinoma/metabolism ; Carcinoma/pathology* ; Cell Line, Tumor ; Humans ; Kangai-1 Protein/biosynthesis ; Kangai-1 Protein/genetics ; Kangai-1 Protein/physiology* ; Lung Neoplasms/enzymology* ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology* ; Matrix Metalloproteinase 9/genetics ; Matrix Metalloproteinase 9/metabolism* ; Matrix Metalloproteinase Inhibitors* ; Neoplasm Invasiveness/pathology ; Neoplasm Invasiveness/prevention & control ; RNA, Messenger/metabolism ; Tissue Inhibitor of Metalloproteinase-1/genetics ; Tissue Inhibitor of Metalloproteinase-1/physiology* ; Transfection ; Up-Regulation/physiology*
Keywords
H1299 non-small cell lung carcinoma cells ; KAI1/CD82 ; MMP9 ; TIMP1 ; Tumor invasiveness
Abstract
We conducted a study on the mechanism of KAI1/CD82-mediated suppression of tumor invasiveness and metastasis, and examined its effect on MMP-9 activity and the TIMP1 levels in H1299 human non-small cell lung carcinoma cells. The H1299 human lung carcinoma cells were transfected with pcDNA3.1-CD82 and stable transfectant clones that had a high KAI1/CD82 expression were obtained. We performed Western blot analysis, cell invasion assay, gelatin zymography, and RT-PCR to assess the KAI1/CD82 expression and tumor invasiveness, the MMP-9 activity, the MMP-9 mRNA and protein levels, and the TIMP1 levels in the H1299/CD82 transfectant cells and compared the results with those of the control groups. The H1299/CD82 transfectants exhibited significant suppression of cell invasion, reduced MMP9 enzyme activity, elevated MMP9 mRNA and MMP-9 protein levels, and elevated TIMP1 levels. It may be postulated that KAI1/CD82 over-expression in the H1299 non-small cell lung carcinoma cells suppresses the tumor invasiveness and metastatic potential by inducing MMP9 inactivation via the up-regulation of TIMP1.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X06002439
DOI
10.1016/j.bbrc.2006.01.153
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Lee, Woon Kyu(이운규)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109795
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