Cited 74 times in
Angiotensin II stimulates the synthesis of vascular endothelial growth factor through the p38 mitogen activated protein kinase pathway in cultured mouse podocytes
DC Field | Value | Language |
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dc.contributor.author | 강신욱 | - |
dc.date.accessioned | 2015-06-10T12:21:05Z | - |
dc.date.available | 2015-06-10T12:21:05Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0952-5041 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109642 | - |
dc.description.abstract | Angiotensin II (Ang-II) and vascular endothelial growth factor (VEGF) have an important role in the pathogenesis of diabetic nephropathy, but the signaling cascade of VEGF regulation in response to Ang-II in podocytes is largely unknown. In these experiments, we looked at the effect of Ang-II on the production of VEGF, and investigated whether VEGF production depends on the p38 mitogen activated protein kinase (MAPK) pathway in cultured mouse podocytes. Incubation of podocytes with Ang-II induced a rapid increase in VEGF mRNA expression and protein synthesis as well as its transcriptional activity in an Ang-II dose-dependent manner. To further define the role of angiotensin type 1 (AT1) and type 2 (AT2) receptors involved in Ang-II-mediated VEGF synthesis, the effects of selective AT1 and AT2 receptor antagonists were evaluated. Prior treatment with losartan significantly inhibited VEGF mRNA and protein synthesis induced by Ang-II, which suggests that the AT1 receptor is involved in Ang-II-mediated VEGF synthesis. Furthermore, stimulation of the cells with Ang-II increased both phosphorylation of p38 MAPK and MAP kinase kinase 3/6 (MKK3/6). Additionally, Ang-II enhanced the DNA binding activity to cAMP response element binding protein (CREB) and phosphorylation of CREB. In addition, to investigate the role of p38 MAPK in Ang-II-induced VEGF synthesis, podocytes were pretreated with or without the p38 MAPK inhibitor, SB203580 for 24 h to observe whether Ang-II-mediated VEGF synthesis was inhibited by blocking p38 MAPK. The addition of SB203580 led to a marked inhibition of the increased VEGF mRNA and protein production induced by Ang-II in a dose-dependent manner. Taken together, these results suggest that Ang-II stimulates the synthesis of VEGF in podocytes and the production of VEGF induced by Ang-II is mediated, in part, through the activation of the p38 MAPK pathway. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 377~388 | - |
dc.relation.isPartOf | JOURNAL OF MOLECULAR ENDOCRINOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Angiotensin II/pharmacology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cyclic AMP Response Element-Binding Protein/metabolism | - |
dc.subject.MESH | Electrophoretic Mobility Shift Assay | - |
dc.subject.MESH | Enzyme Activation/drug effects | - |
dc.subject.MESH | Imidazoles/pharmacology | - |
dc.subject.MESH | MAP Kinase Kinase 3/metabolism | - |
dc.subject.MESH | MAP Kinase Kinase 6/metabolism | - |
dc.subject.MESH | MAP Kinase Signaling System*/drug effects | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Podocytes/drug effects* | - |
dc.subject.MESH | Podocytes/metabolism* | - |
dc.subject.MESH | Protein Kinase Inhibitors/pharmacology | - |
dc.subject.MESH | Pyridines/pharmacology | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Response Elements | - |
dc.subject.MESH | Transcriptional Activation/drug effects | - |
dc.subject.MESH | Up-Regulation/drug effects | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/biosynthesis* | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/genetics | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/metabolism* | - |
dc.title | Angiotensin II stimulates the synthesis of vascular endothelial growth factor through the p38 mitogen activated protein kinase pathway in cultured mouse podocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Young Sun Kang | - |
dc.contributor.googleauthor | Yun Gyu Park | - |
dc.contributor.googleauthor | Bo Kyung Kim | - |
dc.contributor.googleauthor | Sang Youb Han | - |
dc.contributor.googleauthor | Yi Hwa Jee | - |
dc.contributor.googleauthor | Kum Hyun Han | - |
dc.contributor.googleauthor | Mi Hwa Lee | - |
dc.contributor.googleauthor | Hye Kyoung Song | - |
dc.contributor.googleauthor | Dae Ryong Cha | - |
dc.contributor.googleauthor | Shin Wook Kang | - |
dc.contributor.googleauthor | Dae Suk Han | - |
dc.identifier.doi | 10.1677/jme.1.02033 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00053 | - |
dc.relation.journalcode | J01606 | - |
dc.identifier.eissn | 1479-6813 | - |
dc.identifier.pmid | 16595708 | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | Kang, Shin Wook | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 36 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 377 | - |
dc.citation.endPage | 388 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MOLECULAR ENDOCRINOLOGY, Vol.36(2) : 377-388, 2006 | - |
dc.identifier.rimsid | 38889 | - |
dc.type.rims | ART | - |
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