480 598

Cited 65 times in

Oncogenic T-antigen of JC virus is present frequently in human gastric cancers

DC Field Value Language
dc.contributor.author김태일-
dc.contributor.author신성관-
dc.date.accessioned2015-06-10T12:15:00Z-
dc.date.available2015-06-10T12:15:00Z-
dc.date.issued2006-
dc.identifier.issn0008-543X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/109459-
dc.description.abstractBACKGROUND: JC virus (JCV) is a polyomavirus that commonly infects humans and is the causative agent of progressive multifocal leukoencephalopathy in immune-compromised patients. An association between JCV and human cancers long has been suspected, because this virus induces brain tumors in several animal models. The oncogenic potential of JCV is mediated by a transforming protein, the T-antigen (T-Ag), which is a multifunctional protein that transforms cells through interactions with various growth-regulatory genes, including p53 and pRb, and by stabilizing beta-catenin. Previously, the laboratory at the authors' institution demonstrated that JCV is present frequently in the human gastrointestinal tract and may play a role in colorectal carcinogenesis. However, to date, no studies have determined whether JCV sequences are present specifically in gastric cancers. The current study was designed to investigate whether JCV sequences and expression are found in human gastric cancers. METHODS: DNA was extracted from 23 paraffin embedded and 14 frozen gastric cancer specimens. For the detection of JCV gene sequences, polymerase chain reaction amplifications were performed using gene-specific primers for T-Ag, VP-1 (a JCV capsid gene), and the viral regulatory region (or transcriptional control region). Immunohistochemical staining was performed with an anti-T-Ag monoclonal antibody to detect protein expression. RESULTS: Twenty-one of 37 gastric cancers (57%) harbored JCV T-Ag sequences, and 13 of 37 gastric cancers (30%) contained VP-1 sequences. T-Ag sequences also were found in adjacent nonneoplastic mucosa. In addition, JCV regulatory region sequences were present frequently in gastric cancers and adjacent nonneoplastic mucosa. T-Ag protein expression was found in 9 of 23 gastric cancers (39%), whereas no expression was observed in any of the nonneoplastic tissues. CONCLUSIONS: To the authors' knowledge, this is the first demonstration of the presence of JCV T-Ag expression in human gastric cancers. These findings suggest a possible role for this polyomavirus in gastric carcinogenesis.-
dc.description.statementOfResponsibilityopen-
dc.format.extent481~488-
dc.relation.isPartOfCANCER-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAntigens, Polyomavirus Transforming/analysis-
dc.subject.MESHAntigens, Polyomavirus Transforming/genetics*-
dc.subject.MESHAntigens, Polyomavirus Transforming/immunology-
dc.subject.MESHAntigens, Polyomavirus Transforming/metabolism-
dc.subject.MESHChromosomal Instability-
dc.subject.MESHHumans-
dc.subject.MESHJC Virus/genetics*-
dc.subject.MESHJC Virus/immunology-
dc.subject.MESHPolymerase Chain Reaction-
dc.subject.MESHStomach Neoplasms/chemistry-
dc.subject.MESHStomach Neoplasms/genetics-
dc.subject.MESHStomach Neoplasms/virology*-
dc.titleOncogenic T-antigen of JC virus is present frequently in human gastric cancers-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorSung Kwan Shin-
dc.contributor.googleauthorMei-Shu Li-
dc.contributor.googleauthorFlorentine Fuerst-
dc.contributor.googleauthorErin Hotchkiss-
dc.contributor.googleauthorRichard Meyer-
dc.contributor.googleauthorTae Kim II-
dc.contributor.googleauthorAjay Goel-
dc.contributor.googleauthorC. Richard Boland-
dc.identifier.doi10.1002/cncr.22028-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01079-
dc.contributor.localIdA02112-
dc.relation.journalcodeJ00434-
dc.identifier.eissn1097-0142-
dc.identifier.pmid16795066-
dc.subject.keywordJC virus-
dc.subject.keywordpolyomavirus-
dc.subject.keywordT‐antigen-
dc.subject.keywordgastric cancer-
dc.subject.keywordchromosomal instability-
dc.contributor.alternativeNameKim, Tae Il-
dc.contributor.alternativeNameShin, Sung Kwan-
dc.contributor.affiliatedAuthorKim, Tae Il-
dc.contributor.affiliatedAuthorShin, Sung Kwan-
dc.rights.accessRightsfree-
dc.citation.volume107-
dc.citation.number3-
dc.citation.startPage481-
dc.citation.endPage488-
dc.identifier.bibliographicCitationCANCER, Vol.107(3) : 481-488, 2006-
dc.identifier.rimsid57406-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.