Cited 11 times in
Excretory-Secretory Products Produced by Paragonimus westermani Differentially Regulate the Nitric Oxide Production and Viability of Microglial Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신명헌 | - |
dc.date.accessioned | 2015-06-10T12:10:56Z | - |
dc.date.available | 2015-06-10T12:10:56Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1018-2438 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109337 | - |
dc.description.abstract | BACKGROUND: Tissue-invading helminth parasites secrete a large amount of cysteine proteases that may play critical roles in tissue invasion and immune modulation. However, roles of excretory-secretory products (ESP) secreted by Paragonimus westermani in the activation and death of microglial cells in brain are poorly understood. OBJECTIVES: In the present study, we investigated whether ESP could regulate microglial nitric oxide (NO) production and viability. METHODS: The NO production and cell viability were assessed by respectively measuring the formation of nitrite and the release of lactate dehyrogenase. RESULTS: At a low (0.2 microg/ml) concentration, ESP significantly stimulated NO production with no apparent cell injury or death in cultured microglial cells. However, at high (> or =2 microg/ml) concentrations, ESP induced severe cell death. Inhibition of inducible NO synthase significantly reduced the NO productivity, but not cytotoxicity, of ESP. Similarly, inhibitors of the extracellular signal-regulated kinase, p38 and nuclear factor kappa B also blocked only the NO productivity of ESP. Interestingly, heat inactivation did not hamper the ability of ESP to stimulate microglial NO production. Similarly, pretreatment with thiol-crosslinking reagents dramatically reduced both proteolytic activity and cytotoxicity of ESP, but did not alter NO production in microglial cells. Interestingly, although cysteine protease competitive inhibitors and thiol-alkylating reagents markedly reduced the proteolytic activity of ESP, they did not influence the NO productivity and cytotoxicity of ESP. CONCLUSION: The present results indicate that the NO production and cytotoxicity by ESP may be differentially regulated via unknown mechanisms, not related with cysteine protease activity. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 16~24 | - |
dc.relation.isPartOf | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Biological Factors/metabolism* | - |
dc.subject.MESH | Biological Factors/pharmacology* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Hot Temperature | - |
dc.subject.MESH | L-Lactate Dehydrogenase/analysis | - |
dc.subject.MESH | L-Lactate Dehydrogenase/metabolism | - |
dc.subject.MESH | Microglia/metabolism* | - |
dc.subject.MESH | Microglia/pathology* | - |
dc.subject.MESH | Necrosis/pathology* | - |
dc.subject.MESH | Nitrates/analysis | - |
dc.subject.MESH | Nitrates/metabolism | - |
dc.subject.MESH | Nitric Oxide/metabolism* | - |
dc.subject.MESH | Paragonimus westermani/metabolism* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.title | Excretory-Secretory Products Produced by Paragonimus westermani Differentially Regulate the Nitric Oxide Production and Viability of Microglial Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Jin Y. | - |
dc.contributor.googleauthor | Lee J.-C. | - |
dc.contributor.googleauthor | Choi I.Y. | - |
dc.contributor.googleauthor | Kim E.A. | - |
dc.contributor.googleauthor | Shin M.H. | - |
dc.contributor.googleauthor | Kim W.-K. | - |
dc.identifier.doi | 10.1159/000089518 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02099 | - |
dc.relation.journalcode | J01074 | - |
dc.identifier.eissn | 1423-0097 | - |
dc.identifier.pmid | 16272822 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/89518 | - |
dc.contributor.alternativeName | Shin, Myeong Heon | - |
dc.contributor.affiliatedAuthor | Shin, Myeong Heon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 139 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 16 | - |
dc.citation.endPage | 24 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.139(1) : 16-24, 2006 | - |
dc.identifier.rimsid | 52289 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.