Cited 7 times in
9-cis retinoic acid induces insulin-like growth factor binding protein-3 through DR-8 retinoic acid responsive elements
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김성규 | - |
dc.contributor.author | 김세규 | - |
dc.contributor.author | 김형중 | - |
dc.contributor.author | 장윤수 | - |
dc.contributor.author | 장준 | - |
dc.contributor.author | 조재용 | - |
dc.contributor.author | 안철민 | - |
dc.date.accessioned | 2015-06-10T12:00:21Z | - |
dc.date.available | 2015-06-10T12:00:21Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1538-4047 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109017 | - |
dc.description.abstract | Retinoic acids, which have shown potential chemopreventive and therapeutic activities for several neoplastic diseases in vitro, modulate the growth-promoting and anti-apoptotic activities of insulin-like growth factors (IGFs), in part by influencing the expression of insulin-like growth factor binding protein-3 (IGFBP-3). This study sought to investigate the effect of 9-cis retinoic acid (9cRA) on the expression of IGFBP-3 and the underlying mechanisms involving retinoic acid receptor-beta (RAR-beta). The pharmacologic activity of 9cRA was characterized by monitoring target modulation as well as by evaluating the underlying mechanisms in NSCLC cells. Treatment of 9cRA inhibited proliferation of a part of NSCLC cell lines including H460 cells in clinically-achievable concentrations and induced IGFBP-3 expression in dose- and time-dependent manners. Transient transfection with a reporter constructs driven by the human IGFBP-3 gene promoter indicated that 9cRA induces gene expression via the -534 to -445 region (relative to translation start site) of the IGFBP-3 promoter. Unilateral deletion and site-directed mutagenesis identified a retinoic acid responsive element (RARE), a direct repeat of two GGGTCA-related hexanucleotides separated by just 8 bp (DR-8-type response element). A cotransfection assay with a RAR-beta expression vector potentiated (and with siRNA for RAR-beta, diminished) the effect of 9cRA on IGFBP-3 expression. IGFBP-3 gene expression by 9cRA is mediated by a distinct DR-8 RARE located in the proximal region of the IGFBP promoter and involves the RAR-beta, a putative tumor suppressor in NSCLC. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | CANCER BIOLOGY & THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Alitretinoin | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cloning, Molecular | - |
dc.subject.MESH | Connective Tissue Growth Factor | - |
dc.subject.MESH | DNA Primers | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immediate-Early Proteins/genetics* | - |
dc.subject.MESH | Insulin-Like Growth Factor Binding Protein 3/genetics* | - |
dc.subject.MESH | Insulin-Like Growth Factor Binding Protein 4/genetics* | - |
dc.subject.MESH | Insulin-Like Growth Factor Binding Protein 5/genetics* | - |
dc.subject.MESH | Insulin-Like Growth Factor Binding Protein 6/genetics* | - |
dc.subject.MESH | Insulin-Like Growth Factor Binding Proteins/genetics* | - |
dc.subject.MESH | Intercellular Signaling Peptides and Proteins/genetics* | - |
dc.subject.MESH | Plasmids | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Tretinoin/physiology* | - |
dc.title | 9-cis retinoic acid induces insulin-like growth factor binding protein-3 through DR-8 retinoic acid responsive elements | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Yoon Soo Chang | - |
dc.contributor.googleauthor | Jae Yong Cho | - |
dc.contributor.googleauthor | Hyun A. Cho | - |
dc.contributor.googleauthor | Hyung Jung Kim | - |
dc.contributor.googleauthor | Joon Chang | - |
dc.contributor.googleauthor | Chul Min Ahn | - |
dc.contributor.googleauthor | Sung Kyu Kim | - |
dc.contributor.googleauthor | Se Kyu Kim | - |
dc.identifier.doi | 10.4161/cbt.5.6.2658 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00564 | - |
dc.contributor.localId | A00602 | - |
dc.contributor.localId | A01158 | - |
dc.contributor.localId | A02269 | - |
dc.contributor.localId | A03456 | - |
dc.contributor.localId | A03472 | - |
dc.contributor.localId | A03899 | - |
dc.relation.journalcode | J00435 | - |
dc.identifier.eissn | 1555-8576 | - |
dc.identifier.pmid | 16760641 | - |
dc.subject.keyword | insulin-like growth factor | - |
dc.subject.keyword | insulin-like growth factor binding protein-3 | - |
dc.subject.keyword | 9-cis retinoic acids | - |
dc.subject.keyword | retinoic acid receptor-β | - |
dc.subject.keyword | non-small cell lung cancer | - |
dc.contributor.alternativeName | Kim, Sung Kyu | - |
dc.contributor.alternativeName | Kim, Se Kyu | - |
dc.contributor.alternativeName | Kim, Hyung Jung | - |
dc.contributor.alternativeName | Ahn, Chul Min | - |
dc.contributor.alternativeName | Chang, Yoon Soo | - |
dc.contributor.alternativeName | Chang, Joon | - |
dc.contributor.alternativeName | Cho, Jae Yong | - |
dc.contributor.affiliatedAuthor | Kim, Sung Kyu | - |
dc.contributor.affiliatedAuthor | Kim, Se Kyu | - |
dc.contributor.affiliatedAuthor | Kim, Hyung Jung | - |
dc.contributor.affiliatedAuthor | Ahn, Chul Min | - |
dc.contributor.affiliatedAuthor | Chang, Yoon Soo | - |
dc.contributor.affiliatedAuthor | Chang, Joon | - |
dc.contributor.affiliatedAuthor | Cho, Jae Yong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 5 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 586 | - |
dc.citation.endPage | 592 | - |
dc.identifier.bibliographicCitation | CANCER BIOLOGY & THERAPY, Vol.5(6) : 586-592, 2006 | - |
dc.identifier.rimsid | 53714 | - |
dc.type.rims | ART | - |
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