Cited 21 times in
Evidence for alternative candidate genes near RB1 involved in clonal expansion of in situ urothelial neoplasia
DC Field | Value | Language |
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dc.contributor.author | 윤동섭 | - |
dc.contributor.author | 정준 | - |
dc.date.accessioned | 2015-06-10T11:57:22Z | - |
dc.date.available | 2015-06-10T11:57:22Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0023-6837 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108925 | - |
dc.description.abstract | In this paper, we present whole-organ histologic and genetic mapping studies using hypervariable DNA markers on chromosome 13 and then integrate the recombination- and single-nucleotide polymorphic sites (SNPs)-based deletion maps with the annotated genome sequence. Using bladders resected from patients with invasive urothelial carcinoma, we studied allelic patterns of 40 microsatellite markers mapping to all regions of chromosome 13 and 79 SNPs located within the 13q14 region containing the RB1 gene. A whole-organ histologic and genetic mapping strategy was used to identify the evolution of allelic losses on chromosome 13 during the progression of bladder neoplasia. Markers mapping to chromosomal regions involved in clonal expansion of preneoplastic intraurothelial lesions were subsequently tested in 25 tumors and 21 voided urine samples of patients with bladder cancer. Four clusters of allelic losses mapping to distinct regions of chromosome 13 were identified. Markers mapping to the 13q14 region that is flanked by D13S263 and D13S276, which contains the RB1 gene, showed allelic losses associated with early clonal expansion of intraurothelial neoplasia. Such losses could be identified in approximately 32% bladder tumor tissue samples and 38% of voided urines from patients with bladder cancer. The integration of distribution patterns of clonal allelic losses revealed by the microsatellite markers with those obtained by genotyping of SNPs disclosed that the loss within an approximately 4-Mb segment centered around RB1 may represent an incipient event in bladder neoplasia. However, the inactivation of RB1 occurred later and was associated with the onset of severe dysplasia/carcinoma in situ. Our studies provide evidence for the presence of critical alternative candidate genes mapping to the 13q14 region that are involved in clonal expansion of neoplasia within the bladder antecedent to the inactivation of the RB1 gene. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 175~190 | - |
dc.relation.isPartOf | LABORATORY INVESTIGATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Aged, 80 and over | - |
dc.subject.MESH | Alleles | - |
dc.subject.MESH | Chromosome Mapping | - |
dc.subject.MESH | Chromosomes, Human, Pair 13 | - |
dc.subject.MESH | DNA Methylation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Polymorphism, Single Nucleotide | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Recombination, Genetic | - |
dc.subject.MESH | Retinoblastoma Protein/genetics* | - |
dc.subject.MESH | Urinary Bladder Neoplasms/genetics* | - |
dc.subject.MESH | Urinary Bladder Neoplasms/pathology | - |
dc.title | Evidence for alternative candidate genes near RB1 involved in clonal expansion of in situ urothelial neoplasia | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | Mi-Sook Kim | - |
dc.contributor.googleauthor | Joon Jeong | - |
dc.contributor.googleauthor | Tadeusz Majewski | - |
dc.contributor.googleauthor | Andrzej Kram | - |
dc.contributor.googleauthor | Dong-Sup Yoon | - |
dc.contributor.googleauthor | Ruo-Dan Zhang | - |
dc.contributor.googleauthor | Jun-Zhi Li | - |
dc.contributor.googleauthor | Konrad Ptaszynski | - |
dc.contributor.googleauthor | Tang C Kuang | - |
dc.contributor.googleauthor | Jain-Hua Zhou | - |
dc.contributor.googleauthor | Ubaradka G Sathyanarayana | - |
dc.contributor.googleauthor | Tomasz Tuziak | - |
dc.contributor.googleauthor | Dennis A Johnston | - |
dc.contributor.googleauthor | Herbert B Grossman | - |
dc.contributor.googleauthor | Adi F Gazdar | - |
dc.contributor.googleauthor | Steven E Scherer | - |
dc.contributor.googleauthor | William F Benedict | - |
dc.contributor.googleauthor | Bogdan Czerniak | - |
dc.identifier.doi | 10.1038/labinvest.3700378 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02548 | - |
dc.contributor.localId | A03727 | - |
dc.relation.journalcode | J02150 | - |
dc.identifier.eissn | 1530-0307 | - |
dc.identifier.pmid | 16402033 | - |
dc.contributor.alternativeName | Yoon, Dong Sup | - |
dc.contributor.alternativeName | Jeong, Joon | - |
dc.contributor.affiliatedAuthor | Yoon, Dong Sup | - |
dc.contributor.affiliatedAuthor | Jeong, Joon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 86 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 175 | - |
dc.citation.endPage | 190 | - |
dc.identifier.bibliographicCitation | LABORATORY INVESTIGATION, Vol.86(2) : 175-190, 2006 | - |
dc.identifier.rimsid | 50344 | - |
dc.type.rims | ART | - |
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