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Expression of tumor necrosis factor alpha and matrix metalloproteinase-9 in surgically induced necrotizing scleritis

 Kyoung Yul Seo  ;  Hyung Keun Lee  ;  Eung Kweon Kim  ;  Joon H. Lee 
 Ophthalmic Research, Vol.38(2) : 66-70, 2006 
Journal Title
 Ophthalmic Research 
Issue Date
Aged ; Biomarkers/metabolism ; Blotting, Western ; Female ; Follow-Up Studies ; Humans ; Matrix Metalloproteinase 9/biosynthesis* ; Middle Aged ; Ophthalmologic Surgical Procedures/adverse effects* ; Postoperative Complications ; Sclera/metabolism ; Scleritis/etiology ; Scleritis/metabolism* ; Scleritis/pathology ; Tears/metabolism ; Tumor Necrosis Factor-alpha/biosynthesis*
MMP-9 ; Necrotizing scleritis ; TNF-α
BACKGROUND: Surgically induced necrotizing scleritis is primarily managed with immunosuppressive regimens. Such treatments can alter inflammatory cytokines and products such as tumor necrosis factor-alpha (TNF-alpha) and matrix metalloproteinase-9 (MMP-9), which are an important proinflammatory cytokine and a tissue-degrading protease involved in necrosis of some ocular tissues. OBJECT: To evaluate TNF-alpha and MMP-9 in the tear or sclera of surgically induced necrotizing scleritis. METHODS: After tear collection from healthy and diseased eyes of 2 patients, immunoblot analysis using monoclonal antibody specific for human TNF-alpha and MMP-9 was performed. In another patient, scleral tissues were obtained during scleral allograft surgery, and the same immunoblot analysis was done. RESULTS: The level of TNF-alpha and MMP-9 was increased in tear fluid from patients compared to that of control volunteers and returned to the control level after treatment. The diseased sclera showed an increased expression of MMP-9 compared to that of the normal donor sclera. CONCLUSIONS: TNF-alpha and MMP-9 may suggest disease activity of surgically induced necrotizing scleritis and can be altered by proper immunosuppressive treatment.
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1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Seo, Kyuong Yul(서경률) ORCID logo https://orcid.org/0000-0002-9855-1980
Lee, Joon Haeng(이준행)
Lee, Hyung Keun(이형근) ORCID logo https://orcid.org/0000-0002-1123-2136
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