Cited 42 times in
Enhancement of antitumor immunity of dendritic cells pulsed with heat-treated tumor lysate in murine pancreatic cancer
DC Field | Value | Language |
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dc.contributor.author | 김한수 | - |
dc.contributor.author | 방승민 | - |
dc.contributor.author | 송시영 | - |
dc.date.accessioned | 2015-06-10T11:53:31Z | - |
dc.date.available | 2015-06-10T11:53:31Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108808 | - |
dc.description.abstract | Cancer vaccines using dendritic cells (DCs) have been shown to induce antitumor immunity and have recently been applied to non-immunogenic cancers, such as pancreatic cancer. In this study, we utilized DCs loaded with heat-treated tumor lysate (HTL-DC) as a vaccine in order to stimulate antitumor immunity in a murine pancreatic cancer model and compared them to DCs loaded with tumor lysate (TL-DC). The poorly immunogenic mouse ductal pancreatic cancer cell line PANC02 with syngeneic mouse strain C57BL/6 was used as a model. Inducible heat shock proteins (HSPs) were significantly increased in HTL (HSP70 and HSP90). Tumor size measurements indicated that HTL-DC induced stronger tumor suppression than unpulsed DC or TL-DC (43% reduction in tumor volume compared to control group). T cell proliferation assay and IFN-γ ELISPOT assay showed that T cell activation increased in the following order: DC < TL-DC < HTL-DC. Furthermore, repeated HTL-DC vaccinations led to higher expansion of IFN-γ-secreting T cells. Cytotoxicity assay revealed that HTL-DC were more efficient in priming PANC02-specific T cells. Our study identifies HTL as an effective source of tumor-associated antigens (TAAs) for pulsing DCs, and demonstrates that HTL-DC can generate a stronger and broader T cell response against fatal cancers, such as pancreatic cancer. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 142~148 | - |
dc.relation.isPartOf | IMMUNOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Dendritic Cells/immunology* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Heat-Shock Proteins/physiology | - |
dc.subject.MESH | Immunotherapy* | - |
dc.subject.MESH | Interferon-gamma/metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Pancreatic Neoplasms/immunology* | - |
dc.subject.MESH | Pancreatic Neoplasms/therapy | - |
dc.subject.MESH | Spleen/cytology | - |
dc.subject.MESH | Spleen/metabolism | - |
dc.subject.MESH | T-Lymphocytes/physiology | - |
dc.subject.MESH | T-Lymphocytes, Cytotoxic/physiology | - |
dc.subject.MESH | Tissue Extracts/immunology | - |
dc.subject.MESH | Tissue Extracts/therapeutic use* | - |
dc.title | Enhancement of antitumor immunity of dendritic cells pulsed with heat-treated tumor lysate in murine pancreatic cancer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Han-Soo Kim | - |
dc.contributor.googleauthor | Yee Shin Choo | - |
dc.contributor.googleauthor | Taeseok Koo | - |
dc.contributor.googleauthor | Seungmin Bang | - |
dc.contributor.googleauthor | Tae Yun Oh | - |
dc.contributor.googleauthor | Jing Wen | - |
dc.contributor.googleauthor | Si Young Song | - |
dc.identifier.doi | 10.1016/j.imlet.2005.10.021 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01100 | - |
dc.contributor.localId | A01786 | - |
dc.contributor.localId | A02035 | - |
dc.relation.journalcode | J01038 | - |
dc.identifier.eissn | 1879-0542 | - |
dc.identifier.pmid | 16313973 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0165247805003354 | - |
dc.subject.keyword | Dendritic cells | - |
dc.subject.keyword | Immunotherapy | - |
dc.subject.keyword | Tumor lysate | - |
dc.subject.keyword | Murine pancreatic cancer | - |
dc.contributor.alternativeName | Kim, Han Soo | - |
dc.contributor.alternativeName | Bang, Seung Min | - |
dc.contributor.alternativeName | Song, Si Young | - |
dc.contributor.affiliatedAuthor | Kim, Han Soo | - |
dc.contributor.affiliatedAuthor | Bang, Seung Min | - |
dc.contributor.affiliatedAuthor | Song, Si Young | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 103 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 142 | - |
dc.citation.endPage | 148 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY LETTERS, Vol.103(2) : 142-148, 2006 | - |
dc.identifier.rimsid | 57714 | - |
dc.type.rims | ART | - |
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