Cited 109 times in
The Corepressors Silencing Mediator of Retinoid and Thyroid Hormone Receptor and Nuclear Receptor Corepressor Are Involved in Agonist- and Antagonist-Regulated Transcription by Androgen Receptor
DC Field | Value | Language |
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dc.contributor.author | 윤호근 | - |
dc.date.accessioned | 2015-06-10T11:52:54Z | - |
dc.date.available | 2015-06-10T11:52:54Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0888-8809 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108790 | - |
dc.description.abstract | We have investigated the role of corepressors SMRT (silencing mediator of retinoid and thyroid hormone receptor) and N-CoR (nuclear receptor corepressor) in transcriptional regulation by androgen receptor (AR) in the LNCaP prostate cancer cell line. Using specific small interference RNAs to knock down SMRT and/or N-CoR in LNCaP cells, we found that SMRT and N-CoR not only mediate antagonist-dependent inhibition of AR activation but also have a widespread role in suppressing agonist-dependent activation of several AR target genes we have tested, including PSA (prostate-specific antigen), TSC22 (TSC22 domain family member 1), NKX3–1 (NK3 transcription factor locus 1), and B2M(β-2-microglobulin). By sequencing analysis followed by analysis of physical association by chromatin immunoprecipitation assay, we mapped the putative androgen response elements in the NKX3–1 and B2M. Consistent with a role in both antagonist- and agonist-regulated transcription by AR, chromatin immunoprecipitation analysis revealed that both SMRT and N-CoR were recruited by AR to these genes in the presence of either flutamide or R1881. Knocking down SMRT and N-CoR enhanced the recruitment of the coactivators steroid receptor coactivator 1 and p300 by agonist-bound AR and led to increased hyperacetylation of histone H3 and H4, suggesting that the corepressors actively compete with coactivators for binding to agonist-bound AR. Taken together, our data indicate that SMRT and N-CoR corepressors are involved in transcriptional regulation by both agonist- and antagonist-bound AR and regulate the magnitude of hormone response, at least in part, by competing with coactivators. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1048~1060 | - |
dc.relation.isPartOf | MOLECULAR ENDOCRINOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acetylation | - |
dc.subject.MESH | Androgen Receptor Antagonists | - |
dc.subject.MESH | Androgens | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | DNA-Binding Proteins/antagonists & inhibitors | - |
dc.subject.MESH | DNA-Binding Proteins/genetics | - |
dc.subject.MESH | DNA-Binding Proteins/metabolism* | - |
dc.subject.MESH | Enhancer Elements, Genetic | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Histones/metabolism | - |
dc.subject.MESH | Homeodomain Proteins/genetics | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Nuclear Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Nuclear Proteins/genetics | - |
dc.subject.MESH | Nuclear Proteins/metabolism* | - |
dc.subject.MESH | Nuclear Receptor Co-Repressor 1 | - |
dc.subject.MESH | Nuclear Receptor Co-Repressor 2 | - |
dc.subject.MESH | Prostate-Specific Antigen/genetics | - |
dc.subject.MESH | Prostatic Neoplasms/genetics | - |
dc.subject.MESH | Prostatic Neoplasms/metabolism* | - |
dc.subject.MESH | RNA Interference | - |
dc.subject.MESH | RNA, Small Interfering/genetics | - |
dc.subject.MESH | RNA, Small Interfering/pharmacology | - |
dc.subject.MESH | Receptors, Androgen/metabolism* | - |
dc.subject.MESH | Repressor Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Repressor Proteins/genetics | - |
dc.subject.MESH | Repressor Proteins/metabolism* | - |
dc.subject.MESH | Transcription Factors/genetics | - |
dc.subject.MESH | Transcription, Genetic | - |
dc.subject.MESH | beta 2-Microglobulin/genetics | - |
dc.title | The Corepressors Silencing Mediator of Retinoid and Thyroid Hormone Receptor and Nuclear Receptor Corepressor Are Involved in Agonist- and Antagonist-Regulated Transcription by Androgen Receptor | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Ho-Geun Yoon | - |
dc.contributor.googleauthor | Jiemin Wong | - |
dc.identifier.doi | 10.1210/me.2005-0324 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02625 | - |
dc.relation.journalcode | J02257 | - |
dc.identifier.eissn | 1944-9917 | - |
dc.identifier.pmid | 16373395 | - |
dc.identifier.url | http://press.endocrine.org/doi/abs/10.1210/me.2005-0324 | - |
dc.contributor.alternativeName | Yoon, Ho Geun | - |
dc.contributor.affiliatedAuthor | Yoon, Ho Geun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 20 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1048 | - |
dc.citation.endPage | 1060 | - |
dc.identifier.bibliographicCitation | MOLECULAR ENDOCRINOLOGY , Vol.20(5) : 1048-1060, 2006 | - |
dc.identifier.rimsid | 57703 | - |
dc.type.rims | ART | - |
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