Cited 12 times in
Giardia lamblia EB1 is a functional homolog of yeast Bim1p that binds to microtubules
DC Field | Value | Language |
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dc.contributor.author | 김주리 | - |
dc.contributor.author | 박순정 | - |
dc.contributor.author | 용태순 | - |
dc.date.accessioned | 2015-05-19T17:39:48Z | - |
dc.date.available | 2015-05-19T17:39:48Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1383-5769 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108577 | - |
dc.description.abstract | Giardia lamblia, with two nuclei and a distinct polarized morphology, is an interesting organism for investigating how distribution of its microtubule (MT) is controlled during its cell cycle. In this study, we identified the end-binding protein 1 (EB1) of G. lamblia, a well-known microtubule-associated protein that organizes MTs in eukaryotes. Immunofluorescence assays using recombinant EB1 (rEB1)-specific antibodies demonstrated EB1 localization in nuclear membrane as well as in some cytoskeletal structures such as axomenes and median bodies of trophozoites of G. lamblia. Complementation experiments using the BIM1 knock-out mutant of yeast, the yeast homolog of mammalian EB1, showed that giardial EB1 was able to carry out a homologous function in controlling MT dynamics. In addition, rEB1 of G. lamblia co-precipitated with MTs by an in vitro binding assay, thereby demonstrating that G. lamblia EB1 is a MT-associated protein. These results, taken together, suggest that G. lamblia EB1 is a functional homolog of eukaryotic EB1 and is likely to be a determinant for MT distribution. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 465~471 | - |
dc.relation.isPartOf | PARASITOLOGY INTERNATIONAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Cycle Proteins*/chemistry | - |
dc.subject.MESH | Cell Cycle Proteins*/genetics | - |
dc.subject.MESH | Cell Cycle Proteins*/metabolism | - |
dc.subject.MESH | Genetic Complementation Test* | - |
dc.subject.MESH | Giardia lamblia/genetics | - |
dc.subject.MESH | Giardia lamblia/growth & development | - |
dc.subject.MESH | Giardia lamblia/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Microtubule Proteins*/chemistry | - |
dc.subject.MESH | Microtubule Proteins*/genetics | - |
dc.subject.MESH | Microtubule Proteins*/metabolism | - |
dc.subject.MESH | Microtubule-Associated Proteins*/chemistry | - |
dc.subject.MESH | Microtubule-Associated Proteins*/genetics | - |
dc.subject.MESH | Microtubule-Associated Proteins*/metabolism | - |
dc.subject.MESH | Microtubules/metabolism* | - |
dc.subject.MESH | Mutation | - |
dc.subject.MESH | Protozoan Proteins/chemistry | - |
dc.subject.MESH | Protozoan Proteins/genetics | - |
dc.subject.MESH | Protozoan Proteins/metabolism | - |
dc.subject.MESH | Saccharomyces cerevisiae/genetics | - |
dc.subject.MESH | Saccharomyces cerevisiae/growth & development | - |
dc.subject.MESH | Saccharomyces cerevisiae/metabolism* | - |
dc.subject.MESH | Saccharomyces cerevisiae Proteins*/chemistry | - |
dc.subject.MESH | Saccharomyces cerevisiae Proteins*/genetics | - |
dc.subject.MESH | Saccharomyces cerevisiae Proteins*/metabolism | - |
dc.title | Giardia lamblia EB1 is a functional homolog of yeast Bim1p that binds to microtubules | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Juri Kim | - |
dc.contributor.googleauthor | Seobo Sim | - |
dc.contributor.googleauthor | Junwon Kim | - |
dc.contributor.googleauthor | Kiwon Song | - |
dc.contributor.googleauthor | Tai-Soon Yong | - |
dc.contributor.googleauthor | Soon-Jung Park | - |
dc.identifier.doi | 10.1016/j.parint.2008.05.008 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00933 | - |
dc.contributor.localId | A01545 | - |
dc.contributor.localId | A02424 | - |
dc.relation.journalcode | J02936 | - |
dc.identifier.eissn | 1873-0329 | - |
dc.identifier.pmid | 18590831 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1383576908000780 | - |
dc.subject.keyword | Microtubule | - |
dc.subject.keyword | EB1 | - |
dc.subject.keyword | Bim1p | - |
dc.subject.keyword | Microtubule-associated protein | - |
dc.subject.keyword | Giardia lamblia | - |
dc.contributor.alternativeName | Kim, Ju Ri | - |
dc.contributor.alternativeName | Park, Soon Jung | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Kim, Ju Ri | - |
dc.contributor.affiliatedAuthor | Park, Soon Jung | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 57 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 465 | - |
dc.citation.endPage | 471 | - |
dc.identifier.bibliographicCitation | PARASITOLOGY INTERNATIONAL, Vol.57(4) : 465-471, 2008 | - |
dc.identifier.rimsid | 37096 | - |
dc.type.rims | ART | - |
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