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염색체성 AmpC β-lactamase 생성 Enterobacteriaceae 균종에서의 CTX-M형 extended-spectrum β-lactamase 검출과 분자유전학적 특성 규명

Other Titles
 Detection of CTX-M-Type Extended-Spectrum β-Lactamase in Clinical Isolates of Chromosomal AmpC β-Lactamase-Producing Enterobacteriaceae from Korea and Their Molecular Characteristics 
 김창기  ;  염종화  ;  용동은  ;  정석훈  ;  이경원  ;  정윤섭 
 Korean Journal of Clinical Microbiology (대한임상미생물학회지), Vol.11(2) : 90-97, 2008 
Journal Title
 Korean Journal of Clinical Microbiology (대한임상미생물학회지) 
Issue Date
Extended-spectrum β-lactamase ; CTXM ; Genetic characteristics
Background: Clinical isolates of AmpC β-lactamase producing Enterobacteriaceae were evaluated to determine the prevalence of CTX-M extended-spectrum β-lactamases (ESBLs) and their genetic environments. Methods: A total of 250 non-duplicate isolates of Eneterobacter aerogenes, E. cloacae, Citrobacter freundii, Serratia marcescens and Morganella morganii were collected at a Korean hospital. ESBL production was determined by double disk synergy test. For ESBL producers, bla genes were sequenced and blaCTX-M environment was characterized by PCR mapping and sequencing. Results: Among the 250 isolates 29 (11.6%) produced ESBL, and 14 of the 29 isolates produced CTX-M ESBLs, including CTX-M-9 by 8 isolates, CTX-M-3 by 4 isolates, CTX-M-12 by 1 isolate, and CTX-M-14 by 1 isolate. ISEcp1 was present upstream of blaCTX-M-3, 12, and 14. Three of the four CTXM-3 producers had the same genetic environment (pemK-ISEcp1-blaCTX-M-3-orf477-mucA). An IS903-like element was found downstream of blaCTX-M-14. ISCR1 was identified upstream of blaCTX-M-9 and ISCR1 and blaCTX-M-9 were located on sul1-type class 1 integron. The variable region between the 5’-CS and the first 3’-CS contained dfrA16 and aadA2. Its structure was similar to that of In60, but our isolates did not have IS3000 or second 3’-CS. Conclusion: CXT-M type ESBL was prevalent in AmpC β-lactamase-producing Enterobacteriaceae, particularly E. cloacae. blaCTX-M genes were associated with ISEcp1 or ISCR1. This is the first report on the genetic environment of blaCTX-M in Korean isolates.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Research Institute of Bacterial Resistance (세균내성연구소) > 1. Journal Papers
Yonsei Authors
Kim, Chang Ki(김창기)
Yum, Jong Hwa(염종화)
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
Chong, Yun Sop(정윤섭)
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