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Vibrio vulnificus-induced death of Jurkat T-cells requires activation of p38 mitogen-activated protein kinase by NADPH oxidase-derived reactive oxygen species

Authors
 Woo Hyang Kim  ;  Sung Young Goo  ;  Myeong Heon Shin  ;  Se-Jin Chun  ;  Heuiran Lee  ;  Kyu-Ho Lee  ;  Soon-Jung Park 
Citation
 CELLULAR IMMUNOLOGY, Vol.253(1-2) : 81-91, 2008 
Journal Title
 CELLULAR IMMUNOLOGY 
ISSN
 0008-8749 
Issue Date
2008
MeSH
Animals ; Cell Death* ; Enzyme Activation ; Enzyme Inhibitors/metabolism ; Humans ; Jurkat Cells ; Mitogen-Activated Protein Kinase 1/genetics ; Mitogen-Activated Protein Kinase 1/metabolism ; Mitogen-Activated Protein Kinase 3/genetics ; Mitogen-Activated Protein Kinase 3/metabolism ; NADPH Oxidases/metabolism* ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/metabolism ; Onium Compounds/metabolism ; RNA Interference ; Reactive Oxygen Species/metabolism* ; T-Lymphocytes/enzymology ; T-Lymphocytes/microbiology ; T-Lymphocytes/physiology* ; Vibrio vulnificus/pathogenicity* ; p38 Mitogen-Activated Protein Kinases/genetics ; p38 Mitogen-Activated Protein Kinases/metabolism*
Keywords
Vibrio vulnificus ; Jurkat T-cells ; Reactive oxygen species ; Mitogen-activated protein kinases
Abstract
Vibrio vulnificus, a pathogenic bacterium causing primary septicemia, exhibited cytotoxicity towards Jurkat cells of T-lymphocytes through intracellular reactive oxygen species (ROS) production. Pretreatment of Jurkat T-cells with diphenyleneiodonium chloride (DPI) abolished V. vulnificus-induced ROS generation and bacterial ability to cause cell death. Jurkat T-cells expressing dominant-negative protein of Rac subunit of NADPH oxidase (NOX) did not show increased ROS production and cell death by V. vulnificus. Vibrio vulnificus also triggered phosphorylation of mitogen-activated protein kinases (MAPKs) including p38 and ERK1/2 in Jurkat T-cells. Experiments using inhibitors or small interfering RNAs for each MAPK showed that both MAPKs are involved in V. vulnificus-induced cell death. DPI only blocked the phosphorylation of p38 MAPK in Jurkat T-cells exposed by V. vulnificus. This study demonstrates that V. vulnificus induces death of Jurkat T-cells via ROS-dependent activation of p38 MAPK, and that NOX plays a major role in ROS generation in V. vulnificus-exposed cells
Full Text
http://www.sciencedirect.com/science/article/pii/S0008874908000877
DOI
10.1016/j.cellimm.2008.05.003
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Woo Hyang(김우향)
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108280
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