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In situ detection of live cancer cells by using bioprobes based on Au nanoparticles

Authors
 Jaemoon Yang  ;  Kilho Eom  ;  Eun-Kyung Lim  ;  Jinsung Park  ;  Yoonah Kang  ;  Dae Sung Yoon  ;  Sungsoo Na  ;  Eui Kwan Koh  ;  Jin-Suck Suh  ;  Yong-Min Huh  ;  Tae Yun Kwon  ;  Seungjoo Haam 
Citation
 LANGMUIR, Vol.24(21) : 12112-12115, 2008 
Journal Title
 LANGMUIR 
ISSN
 0743-7463 
Issue Date
2008
MeSH
Cell Line, Tumor ; Gold/chemistry* ; Humans ; Metal Nanoparticles* ; Microscopy, Atomic Force ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Molecular Probes* ; Neoplasms/diagnosis* ; Neoplasms/pathology ; Spectrophotometry, Ultraviolet
Keywords
Cell Line, Tumor ; Gold/chemistry* ; Humans ; Metal Nanoparticles* ; Microscopy, Atomic Force ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Molecular Probes* ; Neoplasms/diagnosis* ; Neoplasms/pathology ; Spectrophotometry, Ultraviolet
Abstract
We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals
Full Text
http://pubs.acs.org/doi/abs/10.1021/la802184m
DOI
10.1021/la802184m
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108210
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