Cited 23 times in
Basal c-Jun N-terminal kinases promote mitotic progression through histone H3 phosphorylation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이경희 | - |
dc.date.accessioned | 2015-05-19T17:28:16Z | - |
dc.date.available | 2015-05-19T17:28:16Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1538-4101 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108209 | - |
dc.description.abstract | Phosphorylation of histone H3 at serine 10 (S10) is essential for the onset of mitosis. Here, we show that basal c-Jun N-terminal kinases (JNKs) are required for mitotic histone H3-S10 phosphorylation in human primary fibroblast IMR90 cells. Inhibition of JNKs by specific pharmacologic inhibitors, expression of dominant-negative JNK1 and 2 mutants, or RNAi of JNK1 and 2 prevented phosphorylation of histone H3 at S10 in vivo. The JNK-specific inhibitor SP600125 blocked mitotic entry, as shown by its ability to prevent CDK1 dephosphorylation and cyclin A degradation. Basal JNK phosphorylation increased at G(2)/M phase, although total JNK protein levels remained unchanged. In addition, basal JNKs were localized in nuclei and centrosomes during this time, suggesting that the nuclear localization of JNKs during G(2)/M is tightly coupled with histone H3 phosphorylation. Basal JNKs were able to phosphorylate histone H3 in vitro and coprecipitation of histone H3 and JNKs was only detected at G(2)/M. Taken together, these data strongly suggest that basal JNKs play a key role in controlling histone H3 phosphorylation for mitotic entry at G(2)/M phase. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 216~221 | - |
dc.relation.isPartOf | CELL CYCLE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Anthracenes/pharmacology | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Cell Cycle | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology | - |
dc.subject.MESH | Fibroblasts/cytology | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Histones/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 8/metabolism* | - |
dc.subject.MESH | Mitosis* | - |
dc.subject.MESH | NIH 3T3 Cells | - |
dc.subject.MESH | Phosphorylation | - |
dc.title | Basal c-Jun N-terminal kinases promote mitotic progression through histone H3 phosphorylation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Kyunghee Lee | - |
dc.contributor.googleauthor | Kiwon Song | - |
dc.identifier.doi | 10.4161/cc.7.2.5155 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02662 | - |
dc.relation.journalcode | J00481 | - |
dc.identifier.eissn | 1551-4005 | - |
dc.identifier.pmid | 18256527 | - |
dc.subject.keyword | basal JNKs | - |
dc.subject.keyword | SP600125 | - |
dc.subject.keyword | mitosis | - |
dc.subject.keyword | histone H3 | - |
dc.subject.keyword | IMR90 | - |
dc.contributor.alternativeName | Lee, Kyung Hee | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Hee | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 7 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 216 | - |
dc.citation.endPage | 221 | - |
dc.identifier.bibliographicCitation | CELL CYCLE, Vol.7(2) : 216-221, 2008 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.