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Protection of mice against Mycobacterium tuberculosis infection by immunization with aqueous fraction of Triton X-100-soluble cell wall proteins

Authors
 B.-Y. Jeon  ;  H.-J. Kim  ;  S.-C. Kim  ;  E.-K. Jo  ;  J.-K. Park  ;  T.-H. Paik  ;  S.-J. Kim  ;  S.-N. Cho 
Citation
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY, Vol.67(1) : 18-23, 2008 
Journal Title
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY 
ISSN
 0300-9475 
Issue Date
2008
MeSH
Animals ; Bacterial Proteins/administration & dosage ; Bacterial Proteins/immunology* ; Cattle ; Cell Wall/chemistry ; Cell Wall/immunology* ; Cells, Cultured ; Female ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mycobacterium bovis/immunology ; Mycobacterium tuberculosis/immunology* ; Octoxynol* ; Solubility ; Tuberculosis Vaccines/administration & dosage ; Tuberculosis Vaccines/immunology* ; Tuberculosis, Pulmonary/immunology ; Tuberculosis, Pulmonary/prevention & control*
Abstract
The aqueous fraction of Triton X-100-soluble proteins (TSP-Aq) of Mycobacterium tuberculosis cell wall was reported to stimulate T-cell responses in peripheral blood monocytes from tuberculosis (TB) patients and to induce Th1 cytokines, suggesting presence of protective antigens. In this study, therefore, we examined the protective efficacy of TSP-Aq against M. tuberculosis infection in a mouse model. C57BL/6 mice were immunized with TSP-Aq or culture filtrate proteins (CFP) mixed with incomplete Freund's adjuvant or with BCG followed by i.v. challenge with M. tuberculosis H37Rv. TSP-Aq induced strong interferon-gamma production by spleen cells, and mice immunized with TSP-Aq antigens gave a significant reduction in M. tuberculosis CFU counts by 1.17-1.32 log10 CFU in the lungs and 1.31-2.08 log10 CFU in the spleen from 6 to 28 weeks. The degree of protection offered by TSP-Aq was comparable to that of CFP and of the BCG vaccine. The results demonstrated that the TSP-Aq antigens confer a significant level of protection against the growth of the organism in the lungs and spleen in a mouse model of TB and indicate that TSP contains major protective antigens of M. tuberculosis.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2007.02031.x/abstract
DOI
10.1111/j.1365-3083.2007.02031.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute for Immunology and Immunological Disease (면역질환연구소) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Jeon, Bo Young(전보영)
Cho, Sang Nae(조상래)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108186
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