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Effects of inosine monophosphate dehydrogenase inhibition on high glucose-induced cellular reactive oxygen species in mesangial cells

DC Field Value Language
dc.contributor.author김명수-
dc.contributor.author김유선-
dc.contributor.author주만기-
dc.contributor.author허규하-
dc.date.accessioned2015-05-19T17:24:52Z-
dc.date.available2015-05-19T17:24:52Z-
dc.date.issued2008-
dc.identifier.issn0041-1345-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/108102-
dc.description.abstractMesangial cell extracellular matrix (ECM) synthesis plays an important role in chronic renal diseases including chronic renal allograft dysfunction and diabetic nephropathy. Although inosine monophosphate dehydrogenase 2 (IMPDH2), as a target of mycophenolic acid (MPA), is important for de novo guanosine synthesis in lymphocytes, mesenchymal cells are not wholly dependent on it. To explore the importance of IMPDH2 on the inhibitory effects of MPA in mesangial cells (MC), we compared the effects of MPA and IMPDH2 siRNA on high glucose (HG)-induced fibronectin secretion and cellular reactive oxygen species (ROS). Mouse mesangial cells (MMC) were stimulated with HG (30 mmol/L D-glucose) in the presence or absence of MPA pretreatment or IMPDH2 siRNA transfection. Fibronectin secretion was measured by Western blot analysis, and dichlorofluorescein (DCF)-sensitive cellular ROS assessed by flow cytometry. HG increased fibronectin secretion by 1.8-fold at 24 hours and DCF-sensitive cellular ROS by 1.5-fold at 1 hour. MPA at 10 micromol/L totally inhibited HG-induced fibronectin secretion and cellular ROS in MMC. However, IMPDH2 siRNA only partially suppressed HG-induced fibronectin secretion and cellular ROS. These results suggested that MPA may inhibit HG-induced fibronectin secretion partially through inhibiting cellular ROS and the inhibition of IMPDH2 may be partially involved in the mechanism of MPA.-
dc.description.statementOfResponsibilityopen-
dc.format.extent464~466-
dc.relation.isPartOfTRANSPLANTATION PROCEEDINGS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHGlomerular Mesangium/cytology-
dc.subject.MESHGlomerular Mesangium/drug effects-
dc.subject.MESHGlomerular Mesangium/physiology*-
dc.subject.MESHGlucose/pharmacology*-
dc.subject.MESHIMP Dehydrogenase/antagonists & inhibitors*-
dc.subject.MESHIMP Dehydrogenase/genetics-
dc.subject.MESHMice-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHMycophenolic Acid/pharmacology*-
dc.subject.MESHRNA, Small Interfering/genetics-
dc.subject.MESHReactive Oxygen Species/metabolism*-
dc.titleEffects of inosine monophosphate dehydrogenase inhibition on high glucose-induced cellular reactive oxygen species in mesangial cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorJ.S. Song-
dc.contributor.googleauthorK.H. Huh-
dc.contributor.googleauthorJ. Park-
dc.contributor.googleauthorM.K. Ju-
dc.contributor.googleauthorM.S. Kim-
dc.contributor.googleauthorY.S. Kim-
dc.identifier.doi10.1016/j.transproceed.2008.01.016-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00785-
dc.contributor.localIdA03949-
dc.contributor.localIdA04344-
dc.contributor.localIdA00424-
dc.relation.journalcodeJ02755-
dc.identifier.eissn1873-2623-
dc.identifier.pmid18374103-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0041134508000171-
dc.subject.keywordAnimals-
dc.subject.keywordGlomerular Mesangium/cytology-
dc.subject.keywordGlomerular Mesangium/drug effects-
dc.subject.keywordGlomerular Mesangium/physiology*-
dc.subject.keywordGlucose/pharmacology*-
dc.subject.keywordIMP Dehydrogenase/antagonists & inhibitors*-
dc.subject.keywordIMP Dehydrogenase/genetics-
dc.subject.keywordMice-
dc.subject.keywordMice, Transgenic-
dc.subject.keywordMycophenolic Acid/pharmacology*-
dc.subject.keywordRNA, Small Interfering/genetics-
dc.subject.keywordReactive Oxygen Species/metabolism*-
dc.contributor.alternativeNameKim, Myoung Soo-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameJoo, Man Ki-
dc.contributor.alternativeNameHuh, Kyu Ha-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorJoo, Man Ki-
dc.contributor.affiliatedAuthorHuh, Kyu Ha-
dc.contributor.affiliatedAuthorKim, Myoung Soo-
dc.rights.accessRightsnot free-
dc.citation.volume40-
dc.citation.number2-
dc.citation.startPage464-
dc.citation.endPage466-
dc.identifier.bibliographicCitationTRANSPLANTATION PROCEEDINGS, Vol.40(2) : 464-466, 2008-
dc.identifier.rimsid35183-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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