Cited 6 times in
Circularization of an RNA template via long-range base pairing is critical for hepadnaviral reverse transcription
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김종훈 | - |
dc.date.accessioned | 2015-05-19T17:22:41Z | - |
dc.date.available | 2015-05-19T17:22:41Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0042-6822 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108032 | - |
dc.description.abstract | Although an overall genetic strategy for hepadnaviral reverse transcription has been established, the mechanism that underlies the minus-strand transfer is still poorly defined. We and others independently identified a novel cis-acting element, termed beta or varphi, respectively, that is critical for the minus-strand DNA synthesis of hepatitis B virus. A 5'-3', long-range interaction of the RNA template was proposed that involves the 5' epsilon sequence (encapsidation signal) and the 3' beta/varphi sequence. We subjected the hypothesized base pairing to genetic analysis. The data indicated that mutations abrogating the hypothesized base pairing markedly impaired minus-strand DNA synthesis, while compensatory mutations that restored the base pairing rescued the minus-strand DNA synthesis. These results demonstrated the critical role of the 5'-3', long-range interaction in minus-strand DNA synthesis. We speculate that such a long-range interaction may precisely juxtapose a donor to an acceptor during minus-strand transfer | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 362~373 | - |
dc.relation.isPartOf | VIROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Base Pairing* | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | DNA, Circular/biosynthesis | - |
dc.subject.MESH | DNA, Viral/biosynthesis* | - |
dc.subject.MESH | Hepatitis B virus/genetics* | - |
dc.subject.MESH | Hepatitis B virus/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Mutation | - |
dc.subject.MESH | RNA, Viral/genetics* | - |
dc.subject.MESH | Reverse Transcription* | - |
dc.subject.MESH | Templates, Genetic* | - |
dc.title | Circularization of an RNA template via long-range base pairing is critical for hepadnaviral reverse transcription | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anesthesiology (마취통증의학) | - |
dc.contributor.googleauthor | Myeong-Kyun Shin | - |
dc.contributor.googleauthor | Jong-Hoon Kim | - |
dc.contributor.googleauthor | Dong-Kyun Ryu | - |
dc.contributor.googleauthor | Wang-Shick Ryu | - |
dc.identifier.doi | 10.1016/j.virol.2007.09.042 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00930 | - |
dc.relation.journalcode | J02785 | - |
dc.identifier.eissn | 1096-0341 | - |
dc.identifier.pmid | 17988705 | - |
dc.subject.keyword | Hepatitis B virus | - |
dc.subject.keyword | Reverse transcription | - |
dc.subject.keyword | Minus-strand DNA synthesis | - |
dc.contributor.alternativeName | Kim, Jong Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Jong Hoon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 371 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 362 | - |
dc.citation.endPage | 373 | - |
dc.identifier.bibliographicCitation | VIROLOGY, Vol.371(2) : 362-373, 2008 | - |
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