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Circularization of an RNA template via long-range base pairing is critical for hepadnaviral reverse transcription

DC FieldValueLanguage
dc.contributor.author김종훈-
dc.date.accessioned2015-05-19T17:22:41Z-
dc.date.available2015-05-19T17:22:41Z-
dc.date.issued2008-
dc.identifier.issn0042-6822-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/108032-
dc.description.abstractAlthough an overall genetic strategy for hepadnaviral reverse transcription has been established, the mechanism that underlies the minus-strand transfer is still poorly defined. We and others independently identified a novel cis-acting element, termed beta or varphi, respectively, that is critical for the minus-strand DNA synthesis of hepatitis B virus. A 5'-3', long-range interaction of the RNA template was proposed that involves the 5' epsilon sequence (encapsidation signal) and the 3' beta/varphi sequence. We subjected the hypothesized base pairing to genetic analysis. The data indicated that mutations abrogating the hypothesized base pairing markedly impaired minus-strand DNA synthesis, while compensatory mutations that restored the base pairing rescued the minus-strand DNA synthesis. These results demonstrated the critical role of the 5'-3', long-range interaction in minus-strand DNA synthesis. We speculate that such a long-range interaction may precisely juxtapose a donor to an acceptor during minus-strand transfer-
dc.description.statementOfResponsibilityopen-
dc.format.extent362~373-
dc.relation.isPartOfVIROLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBase Pairing*-
dc.subject.MESHBase Sequence-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHDNA, Circular/biosynthesis-
dc.subject.MESHDNA, Viral/biosynthesis*-
dc.subject.MESHHepatitis B virus/genetics*-
dc.subject.MESHHepatitis B virus/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHMutation-
dc.subject.MESHRNA, Viral/genetics*-
dc.subject.MESHReverse Transcription*-
dc.subject.MESHTemplates, Genetic*-
dc.titleCircularization of an RNA template via long-range base pairing is critical for hepadnaviral reverse transcription-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Anesthesiology (마취통증의학)-
dc.contributor.googleauthorMyeong-Kyun Shin-
dc.contributor.googleauthorJong-Hoon Kim-
dc.contributor.googleauthorDong-Kyun Ryu-
dc.contributor.googleauthorWang-Shick Ryu-
dc.identifier.doi10.1016/j.virol.2007.09.042-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00930-
dc.relation.journalcodeJ02785-
dc.identifier.eissn1096-0341-
dc.identifier.pmid17988705-
dc.subject.keywordHepatitis B virus-
dc.subject.keywordReverse transcription-
dc.subject.keywordMinus-strand DNA synthesis-
dc.contributor.alternativeNameKim, Jong Hoon-
dc.contributor.affiliatedAuthorKim, Jong Hoon-
dc.rights.accessRightsfree-
dc.citation.volume371-
dc.citation.number2-
dc.citation.startPage362-
dc.citation.endPage373-
dc.identifier.bibliographicCitationVIROLOGY, Vol.371(2) : 362-373, 2008-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers

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