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Circularization of an RNA template via long-range base pairing is critical for hepadnaviral reverse transcription

Authors
 Myeong-Kyun Shin  ;  Jong-Hoon Kim  ;  Dong-Kyun Ryu  ;  Wang-Shick Ryu 
Citation
 VIROLOGY, Vol.371(2) : 362-373, 2008 
Journal Title
 VIROLOGY 
ISSN
 0042-6822 
Issue Date
2008
MeSH
Base Pairing* ; Base Sequence ; Cell Line, Tumor ; DNA, Circular/biosynthesis ; DNA, Viral/biosynthesis* ; Hepatitis B virus/genetics* ; Hepatitis B virus/metabolism ; Humans ; Molecular Sequence Data ; Mutation ; RNA, Viral/genetics* ; Reverse Transcription* ; Templates, Genetic*
Keywords
Hepatitis B virus ; Reverse transcription ; Minus-strand DNA synthesis
Abstract
Although an overall genetic strategy for hepadnaviral reverse transcription has been established, the mechanism that underlies the minus-strand transfer is still poorly defined. We and others independently identified a novel cis-acting element, termed beta or varphi, respectively, that is critical for the minus-strand DNA synthesis of hepatitis B virus. A 5'-3', long-range interaction of the RNA template was proposed that involves the 5' epsilon sequence (encapsidation signal) and the 3' beta/varphi sequence. We subjected the hypothesized base pairing to genetic analysis. The data indicated that mutations abrogating the hypothesized base pairing markedly impaired minus-strand DNA synthesis, while compensatory mutations that restored the base pairing rescued the minus-strand DNA synthesis. These results demonstrated the critical role of the 5'-3', long-range interaction in minus-strand DNA synthesis. We speculate that such a long-range interaction may precisely juxtapose a donor to an acceptor during minus-strand transfer
Files in This Item:
T200802678.pdf Download
DOI
10.1016/j.virol.2007.09.042
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jong Hoon(김종훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108032
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